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Literature summary extracted from
- Functional expression and characterization of the myrosinase MYR1 from Brassica napus in Saccharomyces cerevisiae (1999), Protein Expr. Purif., 17, 414-420.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.147 | ascorbic acid | activates | Brassica napus |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.147 | expression of myrosinase MYR1 in Saccharomyces cerevisiae. The recombinant enzyme is enzymatically active which shows that the enzyme, which in plant is complex bound with myrosinase-binding proteins and myrosinase-associated proteins, is functional in its free form. Expression of the enzyme is transient and the growth of the yeast cells is significantly reduced during the period of expression of myrosinase | Brassica napus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.147 | 2-deoxy-2-fluoroglucotropaeolin | - |
Brassica napus | |
| 3.2.1.147 | castanospermine | - |
Brassica napus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.147 | Brassica napus | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.147 | p-hydroxybenzylglucosinolate + H2O | - |
Brassica napus | ? | - |
? | |
| 3.2.1.147 | sinigrin + H2O | - |
Brassica napus | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.147 | 36 | 60 | recombinant enzyme | Brassica napus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.147 | 20 | 65 | 20°C: about 85% of maximal activity, 65°C: about 45% of maximal activity, recombinant enzyme | Brassica napus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.147 | 6 | 7 | recombinant enzyme | Brassica napus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.147 | 4.5 | 9 | pH 4.5: about 55% of maximal activity, pH 9.0: about 80% of maximal activity, recombinant enzyme | Brassica napus |
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