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Literature summary extracted from

  • Shikita, M.; Fahey, J.W.; Golden, R.R.; Holtzclaw, W.D.; Talalay, P.
    An unusual case of uncompetitive activation' by ascorbic acid: purification and kinetic properties of a myrosinase from Raphanus sativus seedlings (1999), Biochem. J., 341, 725-732.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.147 ascorbic acid activates Raphanus sativus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.147 glucose very weak inhibition Raphanus sativus
3.2.1.147 SO42-
-
 Raphanus sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.147 0.023
-
 sinigrin in absence of ascorbate Raphanus sativus
3.2.1.147 0.25
-
 sinigrin in presence of 0.5 mM ascorbate Raphanus sativus
3.2.1.147 14
-
 p-nitrophenyl-beta-D-glucopyranoside in absence of ascorbate Raphanus sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.147 61000
-
 1 * 61000 + 1 * 62000, SDS-PAGE Raphanus sativus
3.2.1.147 62000
-
 1 * 61000 + 1 * 62000, SDS-PAGE Raphanus sativus
3.2.1.147 120000
-
 gel filtration Raphanus sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.147 additional information Raphanus sativus the enzyme is normally physically segregated from the glucosinolates, but when the cells are damaged, e.g. during food preparation, mastication or injury by predators such as insects, the enzyme is released and catalyzes their hydrolysis of glucosinolates ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Raphanus sativus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.147
-
 Raphanus sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.147 root
-
 Raphanus sativus
-
3.2.1.147 seedling
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.147 164
-
-
 Raphanus sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 additional information the enzyme is normally physically segregated from the glucosinolates, but when the cells are damaged, e.g. during food preparation, mastication or injury by predators such as insects, the enzyme is released and catalyzes their hydrolysis of glucosinolates Raphanus sativus ?
-
 ?
3.2.1.147 p-nitrophenyl beta-D-glucopyranoside + H2O
-
 Raphanus sativus ?
-
 ?
3.2.1.147 sinigrin + H2O
-
 Raphanus sativus D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.147 dimer 1 * 61000 + 1 * 62000, SDS-PAGE Raphanus sativus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.147 2.11
-
 sinigrin in absence of ascorbate Raphanus sativus
3.2.1.147 287
-
 sinigrin in presence of 0.5 mM ascorbate Raphanus sativus