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Literature summary extracted from

  • James, D.C.; Rossiter, J.T.
    Development and characteristics of myrosinase in Brassica napus during early seedling growth (1991), Physiol. Plant., 82, 163-170.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.147 ascorbic acid activates Brassica napus
3.2.1.147 ascorbic acid maximal activation for myrosinase I, 50fold at 0.3 mM. Maximal activation for myrosinase II, 106fold between 0.3 and 0.5 mM Brassica napus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.147 62000
-
 3 * 62000, myrosinase II, SDS-PAGE Brassica napus
3.2.1.147 75000
-
 2 * 75000, myrosinase I, SDS-PAGE Brassica napus
3.2.1.147 156000
-
 myrosinase I, gel filtration Brassica napus
3.2.1.147 188000
-
 myrosinase II, gel filtration Brassica napus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Brassica napus
-
 L. cv. Bienvenu
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.147 myrosinase I and II Brassica napus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.147 seedling
-
 Brassica napus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.147 114
-
 myrosinase I Brassica napus
3.2.1.147 283
-
 myrosinase II Brassica napus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 benzylglucosinolate + H2O
-
 Brassica napus additional information
-
 ?
3.2.1.147 glucobrassicin + H2O
-
 Brassica napus ?
-
 ?
3.2.1.147 neo-glucobrassicin + H2O
-
 Brassica napus ?
-
 ?
3.2.1.147 phenylethylglucosinolate + H2O
-
 Brassica napus ?
-
 ?
3.2.1.147 progoitrin + H2O
-
 Brassica napus additional information
-
 ?
3.2.1.147 sinigrin + H2O
-
 Brassica napus D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.147 dimer 2 * 75000, myrosinase I, SDS-PAGE Brassica napus
3.2.1.147 trimer 3 * 62000, myrosinase II, SDS-PAGE Brassica napus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.147 5 6 myrosinase I and II Brassica napus