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Literature summary extracted from

  • Petroski, R.J.; Tookey, H.L.
    Interactions of thioglucoside glucohydrolase and epithiospecifier protein of cruciferous plants to form 1-cyanoepithioalkanes (1982), Phytochemistry, 21, 1903-1905.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.147 epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Brassica oleracea
3.2.1.147 epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Brassica rapa
3.2.1.147 epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Sinapis alba
3.2.1.147 epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Crambe hispanica subsp. abyssinica
3.2.1.147 epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Armoracia rusticana
3.2.1.147 epithiospecifier protein non-specific requirement for epithiospecifier protein Crambe hispanica subsp. abyssinica

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Armoracia rusticana
-
 i.e. horseradish
-
3.2.1.147 Brassica oleracea
-
 var. gemmifera i.e. Brussel sprouts
-
3.2.1.147 Brassica oleracea
-
 var. capitata L. f. alba DC i.e. white cabbage
-
3.2.1.147 Brassica rapa
-
 ssp. rapifera, i.e. turnip
-
3.2.1.147 Crambe hispanica subsp. abyssinica
-
-
-
3.2.1.147 Sinapis alba
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.147 leaf
-
 Brassica oleracea
-
3.2.1.147 leaf
-
 Brassica rapa
-
3.2.1.147 root
-
 Sinapis alba
-
3.2.1.147 root
-
 Armoracia rusticana
-
3.2.1.147 seed
-
 Brassica rapa
-
3.2.1.147 seed
-
 Sinapis alba
-
3.2.1.147 seed
-
 Crambe hispanica subsp. abyssinica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 allylglucosinolate + H2O
-
 Brassica oleracea additional information conversion to 1-cyano-2,3-epithiopropane in presence of epithiospecifier protein, conversion to allyl cyanide in absence of epithiospecifier protein ?
3.2.1.147 allylglucosinolate + H2O
-
 Brassica rapa additional information conversion to 1-cyano-2,3-epithiopropane in presence of epithiospecifier protein, conversion to allyl cyanide in absence of epithiospecifier protein ?
3.2.1.147 allylglucosinolate + H2O
-
 Sinapis alba additional information conversion to 1-cyano-2,3-epithiopropane in presence of epithiospecifier protein, conversion to allyl cyanide in absence of epithiospecifier protein ?
3.2.1.147 allylglucosinolate + H2O
-
 Crambe hispanica subsp. abyssinica additional information conversion to 1-cyano-2,3-epithiopropane in presence of epithiospecifier protein, conversion to allyl cyanide in absence of epithiospecifier protein ?
3.2.1.147 allylglucosinolate + H2O
-
 Armoracia rusticana additional information conversion to 1-cyano-2,3-epithiopropane in presence of epithiospecifier protein, conversion to allyl cyanide in absence of epithiospecifier protein ?
3.2.1.147 sinigrin + H2O
-
 Brassica oleracea D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Brassica rapa D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Sinapis alba D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Crambe hispanica subsp. abyssinica D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
3.2.1.147 sinigrin + H2O
-
 Armoracia rusticana D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?