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Literature summary extracted from

  • Harvey, C.L.; Olson, K.C.; Wright, R.
    Further purification and properties of phosphodiesterase from the carrot (1970), Biochemistry, 9, 921-925.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.4.1 analysis useful tool in nucleic acid research, characterization of oligonucleotides and as an aid in sequence analysis Daucus carota

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.1 2-mercaptoethanol
-
 Daucus carota
3.1.4.1 EDTA inactivation reversed by Mg2+, Mn2+, Ca2+, Zn2+ and slightly by Co2+ Daucus carota
3.1.4.1 glutathione
-
 Daucus carota
3.1.4.1 L-cysteine
-
 Daucus carota
3.1.4.1 Reducing agents
-
 Daucus carota
3.1.4.1 terminal 3'-monophosphoryl group of substrates
-
 Daucus carota

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.1 Ca2+
-
 Daucus carota
3.1.4.1 Co2+ slight activation Daucus carota
3.1.4.1 divalent cations required for activity Daucus carota
3.1.4.1 Mg2+
-
 Daucus carota
3.1.4.1 Mn2+
-
 Daucus carota
3.1.4.1 Zn2+
-
 Daucus carota

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.1 98000 115000 gel filtration Daucus carota

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.1 Daucus carota
-
 carrot
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.1
-
 Daucus carota

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.1 root
-
 Daucus carota
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.1 253.3
-
-
 Daucus carota

Storage Stability

EC Number Storage Stability Organism
3.1.4.1 -20°C, 0.01 M Tris-HCl, pH 7.6, 0.5 M KCl Daucus carota

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.1 ADP + H2O pyrophosphatase activity Daucus carota AMP + phosphate
-
 ?
3.1.4.1 ATP + H2O pyrophosphatase activity Daucus carota 5'-AMP + diphosphate
-
 ?
3.1.4.1 DNA + H2O enzyme hydrolyzes single-stranded DNA, no activity with double-stranded DNA Daucus carota DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
 ?
3.1.4.1 additional information
-
 Daucus carota ?
-
 ?
3.1.4.1 NAD+ + H2O pyrophosphatase activity Daucus carota 5'-AMP + NMN
-
 ?
3.1.4.1 oligonucleotides + H2O specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner Daucus carota oligonucleotides(n-1) + 5'-mononucleotides
-
 ?
3.1.4.1 p-nitrophenylthymidine 5'-phosphate + H2O
-
 Daucus carota 5'-TMP + p-nitrophenol
-
 ?
3.1.4.1 poly d(A-T)2 + H2O
-
 Daucus carota ?
-
 ?
3.1.4.1 polynucleotides + H2O specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner Daucus carota polynucleotides(n-1) + 5'-mononucleotides
-
 ?
3.1.4.1 RNA + H2O
-
 Daucus carota RNA(n-1) + nucleoside 5'-phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.1 60
-
 stable at, without loss of activity Daucus carota

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.1 9 9.5
-
 Daucus carota