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Literature summary extracted from

  • Fitt, P.S.; Baddoo, P.
    Separation and purification of the alkaline phosphatase and a phosphodiesterase from Halobacterium cutirubrum (1979), Biochem. J., 181, 347-353.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.1.4.1 (NH4)2SO4 stabilizes Halobacterium salinarum
3.1.4.1 Mn2+ stabilizes Halobacterium salinarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.1 Mn2+ activation Halobacterium salinarum

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.1 Halobacterium salinarum
-
 extremely halophilic bacterium, mixture of 3'- and 5'-exonuclease
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.1 partial, mixture of 3'- and 5'-exonuclease Halobacterium salinarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.1 additional information
-
-
 Halobacterium salinarum

Storage Stability

EC Number Storage Stability Organism
3.1.4.1 4-5°C, dilute solution, loses about 5% of activity per week Halobacterium salinarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.1 bis(p-nitrophenyl) phosphate + H2O
-
 Halobacterium salinarum ?
-
 ?
3.1.4.1 DNA + H2O
-
 Halobacterium salinarum DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
 ?
3.1.4.1 p-nitrophenylthymidine 5'-phosphate + H2O
-
 Halobacterium salinarum 5'-TMP + p-nitrophenol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.1 30
-
-
 Halobacterium salinarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.1 7 9
-
 Halobacterium salinarum