BRENDA - Enzyme Database

Enzymes hydrolyzing ApppA and/or AppppA in higher plants. Purification and some properties of diadenosine triphosphatase, diadenosine tetraphosphatase, and phosphodiesterase from yellow lupin (Lupinus luteus) seeds

Jakubowski, H.; Guranowski, A.; J. Biol. Chem. 258, 9982-9989 (1983)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.1
AMP
product inhibition
Lupinus luteus
3.1.4.1
Co2+
no inhibition
Lupinus luteus
3.1.4.1
EDTA
no inhibition
Lupinus luteus
3.1.4.1
Mn2+
no inhibition
Lupinus luteus
3.1.4.1
additional information
no inhibition by divalent cations, Mg2+ and Ca2+ , or p-chloromercuribenzoate
Lupinus luteus
3.1.4.1
Ni2+
no inhibition
Lupinus luteus
3.1.4.1
Zn2+
no inhibition
Lupinus luteus
3.6.1.29
Ca2+
-
Lupinus luteus
3.6.1.29
Co2+
-
Lupinus luteus
3.6.1.29
Mn2+
-
Lupinus luteus
3.6.1.29
Ni2+
-
Lupinus luteus
3.6.1.29
spermine
-
Lupinus luteus
3.6.1.29
Zn2+
-
Lupinus luteus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.1
0.002
-
diadenosine 5',5'''-P1,P4-tetraphosphate
-
Lupinus luteus
3.1.4.1
0.0022
-
diadenosine 5',5'''-P1,P3-triphosphate
-
Lupinus luteus
3.1.4.1
0.0096
-
NAD+
-
Lupinus luteus
3.6.1.29
0.001
-
P1-P3-bis(5'-adenosyl) triphosphate
-
Lupinus luteus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.1
divalent cations
not required for activity
Lupinus luteus
3.1.4.1
Mg2+
not required for activity
Lupinus luteus
3.6.1.29
Mg2+
required for activity
Lupinus luteus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.1
56000
-
1 * 56000, SDS-PAGE
Lupinus luteus
3.1.4.1
65000
-
gel filtration
Lupinus luteus
3.6.1.29
41000
-
-
Lupinus luteus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.1
diadenosine polyphosphate + H2O
Lupinus luteus
phosphodiesterase can be considered as a catabolic enzyme
AMP
-
Lupinus luteus
?
3.6.1.29
P1-P3-bis(5'-adenosyl) triphosphate + H2O
Lupinus luteus
-
ADP + AMP
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.1
Lupinus luteus
-
yellow lupin
-
3.6.1.29
Lupinus luteus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.1
-
Lupinus luteus
3.6.1.29
-
Lupinus luteus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.1
seed
-
Lupinus luteus
-
3.6.1.29
seed
-
Lupinus luteus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.4.1
0.9
-
-
Lupinus luteus
3.6.1.29
0.07
-
-
Lupinus luteus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.1
3'-p-nitrophenylthymidine 3'-phosphate + H2O
-
208361
Lupinus luteus
3'-TMP + p-nitrophenol
-
-
-
?
3.1.4.1
adenosine 5'-P1-tetraphospho-P4-5'''-adenosine + H2O
-
208361
Lupinus luteus
AMP + ATP
-
208361
Lupinus luteus
?
3.1.4.1
ADP + H2O
-
208361
Lupinus luteus
AMP + phosphate
-
-
-
?
3.1.4.1
ATP + H2O
-
208361
Lupinus luteus
5'-AMP + diphosphate
-
-
-
?
3.1.4.1
bis(p-nitrophenyl) phosphate + H2O
-
208361
Lupinus luteus
?
-
-
-
?
3.1.4.1
diadenosine 5',5'''-P1,P3-triphosphate + H2O
-
208361
Lupinus luteus
AMP + ADP
-
208361
Lupinus luteus
?
3.1.4.1
diadenosine diphosphate + H2O
-
208361
Lupinus luteus
?
-
-
-
?
3.1.4.1
diadenosine polyphosphate + H2O
phosphodiesterase can be considered as a catabolic enzyme
208361
Lupinus luteus
AMP
-
208361
Lupinus luteus
?
3.1.4.1
dinucleoside polyphosphate + H2O
-
208361
Lupinus luteus
?
-
-
-
?
3.1.4.1
DNA + H2O
-
208361
Lupinus luteus
DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
-
-
?
3.1.4.1
FAD + H2O
-
208361
Lupinus luteus
AMP + FMN
-
208361
Lupinus luteus
?
3.1.4.1
additional information
no activity with cAMP
208361
Lupinus luteus
?
-
-
-
-
3.1.4.1
additional information
broad substrate specificity
208361
Lupinus luteus
?
-
-
-
-
3.1.4.1
NAD+ + H2O
-
208361
Lupinus luteus
5'-AMP + NMN
-
-
-
?
3.1.4.1
NADP+ + H2O
-
208361
Lupinus luteus
NAD+ + phosphate
-
-
-
?
3.1.4.1
p-nitrophenylthymidine 5'-phosphate + H2O
-
208361
Lupinus luteus
5'-TMP + p-nitrophenol
-
-
-
?
3.6.1.29
adenosyl-cytosinyl triphosphate + H2O
-
208361
Lupinus luteus
CDP + AMP
-
-
-
?
3.6.1.29
adenosyl-guanosine triphosphate + H2O
-
208361
Lupinus luteus
GDP + AMP
-
-
-
?
3.6.1.29
adenosyl-ribose triphosphate + H2O
-
208361
Lupinus luteus
AMP + ribose diphosphate
-
-
-
?
3.6.1.29
P1-P3-bis(5'-adenosyl) triphosphate + H2O
-
208361
Lupinus luteus
ADP + AMP
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.4.1
monomer
1 * 56000, SDS-PAGE
Lupinus luteus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.1
9
10.5
-
Lupinus luteus
3.6.1.29
5
8
-
Lupinus luteus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.1
5.5
-
above, the activity of phosphodiesterase increases continuously with increasing pH, from zero activity below pH 5.5
Lupinus luteus
3.6.1.29
4.6
10
-
Lupinus luteus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.1
AMP
product inhibition
Lupinus luteus
3.1.4.1
Co2+
no inhibition
Lupinus luteus
3.1.4.1
EDTA
no inhibition
Lupinus luteus
3.1.4.1
Mn2+
no inhibition
Lupinus luteus
3.1.4.1
additional information
no inhibition by divalent cations, Mg2+ and Ca2+ , or p-chloromercuribenzoate
Lupinus luteus
3.1.4.1
Ni2+
no inhibition
Lupinus luteus
3.1.4.1
Zn2+
no inhibition
Lupinus luteus
3.6.1.29
Ca2+
-
Lupinus luteus
3.6.1.29
Co2+
-
Lupinus luteus
3.6.1.29
Mn2+
-
Lupinus luteus
3.6.1.29
Ni2+
-
Lupinus luteus
3.6.1.29
spermine
-
Lupinus luteus
3.6.1.29
Zn2+
-
Lupinus luteus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.1
0.002
-
diadenosine 5',5'''-P1,P4-tetraphosphate
-
Lupinus luteus
3.1.4.1
0.0022
-
diadenosine 5',5'''-P1,P3-triphosphate
-
Lupinus luteus
3.1.4.1
0.0096
-
NAD+
-
Lupinus luteus
3.6.1.29
0.001
-
P1-P3-bis(5'-adenosyl) triphosphate
-
Lupinus luteus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.1
divalent cations
not required for activity
Lupinus luteus
3.1.4.1
Mg2+
not required for activity
Lupinus luteus
3.6.1.29
Mg2+
required for activity
Lupinus luteus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.1
56000
-
1 * 56000, SDS-PAGE
Lupinus luteus
3.1.4.1
65000
-
gel filtration
Lupinus luteus
3.6.1.29
41000
-
-
Lupinus luteus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.1
diadenosine polyphosphate + H2O
Lupinus luteus
phosphodiesterase can be considered as a catabolic enzyme
AMP
-
Lupinus luteus
?
3.6.1.29
P1-P3-bis(5'-adenosyl) triphosphate + H2O
Lupinus luteus
-
ADP + AMP
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.1
-
Lupinus luteus
3.6.1.29
-
Lupinus luteus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.1
seed
-
Lupinus luteus
-
3.6.1.29
seed
-
Lupinus luteus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.4.1
0.9
-
-
Lupinus luteus
3.6.1.29
0.07
-
-
Lupinus luteus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.1
3'-p-nitrophenylthymidine 3'-phosphate + H2O
-
208361
Lupinus luteus
3'-TMP + p-nitrophenol
-
-
-
?
3.1.4.1
adenosine 5'-P1-tetraphospho-P4-5'''-adenosine + H2O
-
208361
Lupinus luteus
AMP + ATP
-
208361
Lupinus luteus
?
3.1.4.1
ADP + H2O
-
208361
Lupinus luteus
AMP + phosphate
-
-
-
?
3.1.4.1
ATP + H2O
-
208361
Lupinus luteus
5'-AMP + diphosphate
-
-
-
?
3.1.4.1
bis(p-nitrophenyl) phosphate + H2O
-
208361
Lupinus luteus
?
-
-
-
?
3.1.4.1
diadenosine 5',5'''-P1,P3-triphosphate + H2O
-
208361
Lupinus luteus
AMP + ADP
-
208361
Lupinus luteus
?
3.1.4.1
diadenosine diphosphate + H2O
-
208361
Lupinus luteus
?
-
-
-
?
3.1.4.1
diadenosine polyphosphate + H2O
phosphodiesterase can be considered as a catabolic enzyme
208361
Lupinus luteus
AMP
-
208361
Lupinus luteus
?
3.1.4.1
dinucleoside polyphosphate + H2O
-
208361
Lupinus luteus
?
-
-
-
?
3.1.4.1
DNA + H2O
-
208361
Lupinus luteus
DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
-
-
?
3.1.4.1
FAD + H2O
-
208361
Lupinus luteus
AMP + FMN
-
208361
Lupinus luteus
?
3.1.4.1
additional information
no activity with cAMP
208361
Lupinus luteus
?
-
-
-
-
3.1.4.1
additional information
broad substrate specificity
208361
Lupinus luteus
?
-
-
-
-
3.1.4.1
NAD+ + H2O
-
208361
Lupinus luteus
5'-AMP + NMN
-
-
-
?
3.1.4.1
NADP+ + H2O
-
208361
Lupinus luteus
NAD+ + phosphate
-
-
-
?
3.1.4.1
p-nitrophenylthymidine 5'-phosphate + H2O
-
208361
Lupinus luteus
5'-TMP + p-nitrophenol
-
-
-
?
3.6.1.29
adenosyl-cytosinyl triphosphate + H2O
-
208361
Lupinus luteus
CDP + AMP
-
-
-
?
3.6.1.29
adenosyl-guanosine triphosphate + H2O
-
208361
Lupinus luteus
GDP + AMP
-
-
-
?
3.6.1.29
adenosyl-ribose triphosphate + H2O
-
208361
Lupinus luteus
AMP + ribose diphosphate
-
-
-
?
3.6.1.29
P1-P3-bis(5'-adenosyl) triphosphate + H2O
-
208361
Lupinus luteus
ADP + AMP
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.4.1
monomer
1 * 56000, SDS-PAGE
Lupinus luteus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.1
9
10.5
-
Lupinus luteus
3.6.1.29
5
8
-
Lupinus luteus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.4.1
5.5
-
above, the activity of phosphodiesterase increases continuously with increasing pH, from zero activity below pH 5.5
Lupinus luteus
3.6.1.29
4.6
10
-
Lupinus luteus