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Literature summary extracted from

  • Jakubowski, H.; Guranowski, A.
    Enzymes hydrolyzing ApppA and/or AppppA in higher plants. Purification and some properties of diadenosine triphosphatase, diadenosine tetraphosphatase, and phosphodiesterase from yellow lupin (Lupinus luteus) seeds (1983), J. Biol. Chem., 258, 9982-9989.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.1 AMP product inhibition Lupinus luteus
3.1.4.1 Co2+ no inhibition Lupinus luteus
3.1.4.1 EDTA no inhibition Lupinus luteus
3.1.4.1 Mn2+ no inhibition Lupinus luteus
3.1.4.1 additional information no inhibition by divalent cations, Mg2+ and Ca2+ , or p-chloromercuribenzoate Lupinus luteus
3.1.4.1 Ni2+ no inhibition Lupinus luteus
3.1.4.1 Zn2+ no inhibition Lupinus luteus
3.6.1.29 Ca2+
-
 Lupinus luteus
3.6.1.29 Co2+
-
 Lupinus luteus
3.6.1.29 Mn2+
-
 Lupinus luteus
3.6.1.29 Ni2+
-
 Lupinus luteus
3.6.1.29 spermine
-
 Lupinus luteus
3.6.1.29 Zn2+
-
 Lupinus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.1 0.002
-
 diadenosine 5',5'''-P1,P4-tetraphosphate
-
 Lupinus luteus
3.1.4.1 0.0022
-
 diadenosine 5',5'''-P1,P3-triphosphate
-
 Lupinus luteus
3.1.4.1 0.0096
-
 NAD+
-
 Lupinus luteus
3.6.1.29 0.001
-
 P1-P3-bis(5'-adenosyl) triphosphate
-
 Lupinus luteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.1 divalent cations not required for activity Lupinus luteus
3.1.4.1 Mg2+ not required for activity Lupinus luteus
3.6.1.29 Mg2+ required for activity Lupinus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.1 56000
-
 1 * 56000, SDS-PAGE Lupinus luteus
3.1.4.1 65000
-
 gel filtration Lupinus luteus
3.6.1.29 41000
-
-
 Lupinus luteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.1 diadenosine polyphosphate + H2O Lupinus luteus phosphodiesterase can be considered as a catabolic enzyme AMP + adenosine polyphosphate-1
-
 ?
3.6.1.29 P1-P3-bis(5'-adenosyl) triphosphate + H2O Lupinus luteus
-
 ADP + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.1 Lupinus luteus
-
 yellow lupin
-
3.6.1.29 Lupinus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.1
-
 Lupinus luteus
3.6.1.29
-
 Lupinus luteus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.1 seed
-
 Lupinus luteus
-
3.6.1.29 seed
-
 Lupinus luteus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.1 0.9
-
-
 Lupinus luteus
3.6.1.29 0.07
-
-
 Lupinus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.1 3'-p-nitrophenylthymidine 3'-phosphate + H2O
-
 Lupinus luteus 3'-TMP + p-nitrophenol
-
 ?
3.1.4.1 adenosine 5'-P1-tetraphospho-P4-5'''-adenosine + H2O
-
 Lupinus luteus AMP + ATP
-
 ?
3.1.4.1 ADP + H2O
-
 Lupinus luteus AMP + phosphate
-
 ?
3.1.4.1 ATP + H2O
-
 Lupinus luteus 5'-AMP + diphosphate
-
 ?
3.1.4.1 bis(p-nitrophenyl) phosphate + H2O
-
 Lupinus luteus ?
-
 ?
3.1.4.1 diadenosine 5',5'''-P1,P3-triphosphate + H2O
-
 Lupinus luteus AMP + ADP
-
 ?
3.1.4.1 diadenosine diphosphate + H2O
-
 Lupinus luteus ?
-
 ?
3.1.4.1 diadenosine polyphosphate + H2O phosphodiesterase can be considered as a catabolic enzyme Lupinus luteus AMP + adenosine polyphosphate-1
-
 ?
3.1.4.1 dinucleoside polyphosphate + H2O
-
 Lupinus luteus ?
-
 ?
3.1.4.1 DNA + H2O
-
 Lupinus luteus DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
-
 ?
3.1.4.1 FAD + H2O
-
 Lupinus luteus AMP + FMN
-
 ?
3.1.4.1 additional information no activity with cAMP Lupinus luteus ?
-
 ?
3.1.4.1 additional information broad substrate specificity Lupinus luteus ?
-
 ?
3.1.4.1 NAD+ + H2O
-
 Lupinus luteus 5'-AMP + NMN
-
 ?
3.1.4.1 NADP+ + H2O
-
 Lupinus luteus NAD+ + phosphate
-
 ?
3.1.4.1 p-nitrophenylthymidine 5'-phosphate + H2O
-
 Lupinus luteus 5'-TMP + p-nitrophenol
-
 ?
3.6.1.29 adenosyl-cytosinyl triphosphate + H2O
-
 Lupinus luteus CDP + AMP
-
 ?
3.6.1.29 adenosyl-guanosine triphosphate + H2O
-
 Lupinus luteus GDP + AMP
-
 ?
3.6.1.29 adenosyl-ribose triphosphate + H2O
-
 Lupinus luteus AMP + ribose diphosphate
-
 ?
3.6.1.29 P1-P3-bis(5'-adenosyl) triphosphate + H2O
-
 Lupinus luteus ADP + AMP
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.1 monomer 1 * 56000, SDS-PAGE Lupinus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.1 9 10.5
-
 Lupinus luteus
3.6.1.29 5 8
-
 Lupinus luteus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.1 5.5
-
 above, the activity of phosphodiesterase increases continuously with increasing pH, from zero activity below pH 5.5 Lupinus luteus
3.6.1.29 4.6 10
-
 Lupinus luteus