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Literature summary extracted from

  • Nojima, S.; Mineki, S.; Iida, M.
    Purification and characterization of extracellular poly(3-hydroxybutyrate) depolymerases produced by Agrobacterium sp. K-03 (1996), J. Ferment. Bioeng., 81, 72-75.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.75 dithiothreitol considerable inactivation Agrobacterium sp.
3.1.1.75 phenylmethanesulfonyl fluoride considerable inactivation Agrobacterium sp.
3.1.1.75 Tween 20 considerable inactivation Agrobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.75 additional information
-
 poly(3-hydroxybutanoate)n 0.018 mg/ml, isoform E1, 0.07 mg/ml, isoform E2 Agrobacterium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.75 extracellular
-
 Agrobacterium sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.75 44000
-
 isofrom E2, gel filtration Agrobacterium sp.
3.1.1.75 46000
-
 isoform E1, gel filtration Agrobacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O Agrobacterium sp.
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O Agrobacterium sp. K-03
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Agrobacterium sp.
-
-
-
3.1.1.75 Agrobacterium sp. K-03
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.75 to homogeneity, 2 isoforms, E1, E2 Agrobacterium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.75 cell culture
-
 Agrobacterium sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 methyl (3R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoate + H2O
-
 Agrobacterium sp. (R)-3-hydroxybutanoic acid + methyl-(R)-3-hydroxybutanoate + (R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoic acid
-
 ?
3.1.1.75 methyl (3R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoate + H2O
-
 Agrobacterium sp. K-03 (R)-3-hydroxybutanoic acid + methyl-(R)-3-hydroxybutanoate + (R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoic acid
-
 ?
3.1.1.75 poly(3-hydroxybutyrate-co-[43%]3-hydroxyvalerate)n + H2O
-
 Agrobacterium sp. poly(3-hydroxybutyrate-co-[43%]3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-[43%]3-hydroxyvalerate)
-
 ?
3.1.1.75 poly(3-hydroxybutyrate-co-[43%]3-hydroxyvalerate)n + H2O
-
 Agrobacterium sp. K-03 poly(3-hydroxybutyrate-co-[43%]3-hydroxyvalerate)n-x + poly(3-hydroxybutyrate-co-[43%]3-hydroxyvalerate)
-
 ?
3.1.1.75 poly(3-hydroxybutyric acid) + H2O
-
 Agrobacterium sp. ?
-
 ?
3.1.1.75 poly(3-hydroxybutyric acid) + H2O
-
 Agrobacterium sp. K-03 ?
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O
-
 Agrobacterium sp. poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O
-
 Agrobacterium sp. K-03 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.75 45
-
-
 Agrobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.75 7.9
-
 isoform E2 Agrobacterium sp.
3.1.1.75 8.1
-
 isoform E1 Agrobacterium sp.