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Literature summary extracted from

  • Sadacco, P.; Nocerino, S.; Dubini-Paglia, E.; Seves, A.; Elegir, G.
    Characterization of a poly(3-hydroxybutyrate) depolymerase from Aureobacterium saperdae: Acive site and kinetics of hydrolysis studies (1997), J. Environ. Polym. Degrad., 5, 57-65.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.75 2-Hydroxy-5-nitrobenzyl bromide complete inactivation Microbacterium saperdae
3.1.1.75 diazo-DL-norleucine methyl ester very sensitive to Microbacterium saperdae
3.1.1.75 diisopropyl fluorophosphate very sensitive to Microbacterium saperdae
3.1.1.75 dithiothreitol rapid inactivation Microbacterium saperdae
3.1.1.75 N-bromosuccinimide complete inactivation Microbacterium saperdae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.75 extracellular
-
 Microbacterium saperdae
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.75 42700
-
 gel filtration Microbacterium saperdae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O Microbacterium saperdae
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x main products: dimer and monomer ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Microbacterium saperdae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.75 to homogeneity, chromatography steps Microbacterium saperdae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.75 cell culture
-
 Microbacterium saperdae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O
-
 Microbacterium saperdae poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x main products: dimer and monomer ?

Subunits

EC Number Subunits Comment Organism
3.1.1.75 monomer 1 * 42700, SDS-PAGE Microbacterium saperdae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.75 8
-
-
 Microbacterium saperdae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.75 3 11 stable between Microbacterium saperdae