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Literature summary extracted from

  • Saegusa, H.; Shiraki, M.; Kanai, C.; Saito, T.
    Cloning of an intracellular poly[D(-)-3-hydroxybutyrate] depolymerase gene from Ralstonia eutropha H16 and characterization of the gene product (2001), J. Bacteriol., 183, 94-100.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.75 expression in Escherichia coli Cupriavidus necator

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.75 intracellular
-
 Cupriavidus necator 5622
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O Cupriavidus necator no hydrolysis of crystalline poly(3-hydroxybutyrate) poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x mainly oligomeric and some monomeric release of D(-)-3-hydroxybutyrate ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 no hydrolysis of crystalline poly(3-hydroxybutyrate) poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x mainly oligomeric and some monomeric release of D(-)-3-hydroxybutyrate ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Cupriavidus necator
-
-
-
3.1.1.75 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.75 cell culture
-
 Cupriavidus necator
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O no hydrolysis of crystalline poly(3-hydroxybutyrate) Cupriavidus necator poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x mainly oligomeric and some monomeric release of D(-)-3-hydroxybutyrate ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O no hydrolysis of crystalline poly(3-hydroxybutyrate) Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x mainly oligomeric and some monomeric release of D(-)-3-hydroxybutyrate ?