EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.75 | 2-propanol | partial inhibition of P(3HV) depolymerase | Paucimonas lemoignei | |
3.1.1.75 | dithioerythritol | complete inhibition of P(3HV) depolymerase at 4 mM, reversible by dithionitrobenzene | Paucimonas lemoignei | |
3.1.1.75 | EDTA | partial inhibition of P(3HV) depolymerase at concentrations higher than 5 mM | Paucimonas lemoignei | |
3.1.1.75 | ethanol | partial inhibition of P(3HV) depolymerase | Paucimonas lemoignei | |
3.1.1.75 | PMSF | partial inhibition at 0.5 mM | Paucimonas lemoignei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.75 | additional information | - |
poly(3-hydroxybutanoate)n | 0.06 mg/ml, P(3HB) depolymerase B, 0.1 mg/ml, P(3HB) depolymerase A | Paucimonas lemoignei | |
3.1.1.75 | additional information | - |
poly(3-hydroxybutanoate)n | values in mg/ml | Paucimonas lemoignei |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.75 | extracellular | - |
Paucimonas lemoignei | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.75 | 53000 | - |
sucrose density gradient centrifugation | Paucimonas lemoignei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | Paucimonas lemoignei | highest activities for poly(3-hydroxybutyrate) depolymerases A and B, low activity for poly(3-hydroxyvaleric acid) depolymerase | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.75 | Paucimonas lemoignei | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.75 | cell culture | - |
Paucimonas lemoignei | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.75 | additional information | - |
19.1 mg poly[(R)-3-hydroxybutanoate]/min * mg protein, P(3HB) depolymerase A, 15.9 mg poly[(R)-3-hydroxybutanoate]/min * mg protein, P(3HB) depolymerase B, 9.27 mg poly(3-hydroxyvaleric acid)/min * mg protein | Paucimonas lemoignei |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.1.75 | -20°C, P(3HV) depolymerase, 1 month | Paucimonas lemoignei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.75 | poly(3-hydroxybutyric acid) + H2O | - |
Paucimonas lemoignei | ? | - |
? | |
3.1.1.75 | poly(3-hydroxyvalerate)n + H2O | highest activity for poly(3-hydroxyvaleric acid) depolymerase, low activity for poly(3-hydroxybutyrate) depolymerases A and B | Paucimonas lemoignei | poly(3-hydroxyvalerate)n-x + poly(3-hydroxyvalerate) | - |
? | |
3.1.1.75 | poly[(R)-3-hydroxybutanoate]n + H2O | highest activities for poly(3-hydroxybutyrate) depolymerases A and B, low activity for poly(3-hydroxyvaleric acid) depolymerase | Paucimonas lemoignei | poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.75 | monomer | 1 * 53000, SDS-PAGE | Paucimonas lemoignei |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.75 | P(3HB) depolymerase A | - |
Paucimonas lemoignei |
3.1.1.75 | P(3HB) depolymerase B | - |
Paucimonas lemoignei |
3.1.1.75 | P(3HV) depolymerase | - |
Paucimonas lemoignei |
3.1.1.75 | poly(3-hydroxyvaleric acid) depolymerase | - |
Paucimonas lemoignei |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.75 | 55 | - |
P(3HV) depolymerase | Paucimonas lemoignei |
3.1.1.75 | 65 | - |
P(3HB) depolymerase A | Paucimonas lemoignei |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.75 | 20 | - |
P(3HV) depolymerase: 25% loss of activity within 2 months | Paucimonas lemoignei |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.75 | 8 | - |
P(3HB) depolymerase A, P(3HV) depolymerase | Paucimonas lemoignei |