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Literature summary extracted from

  • Ohura, T.; Kasuya, K.I.; Doi, Y.
    Cloning and characterization of the polyhydroxybutyrate depolymerase gene of Pseudomonas stutzeri and analysis of the function of substrate-binding domains (1999), Appl. Environ. Microbiol., 65, 189-197.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.75 phaZ PSTgene product, expression in Escherichia coli Stutzerimonas stutzeri

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.75 additional information construction of several GST-fusion domains, analysis of substrate-binding domains Stutzerimonas stutzeri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.75 extracellular
-
 Stutzerimonas stutzeri
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O Stutzerimonas stutzeri
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Stutzerimonas stutzeri
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.75 cell culture
-
 Stutzerimonas stutzeri
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 (R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoic acid + H2O
-
 Stutzerimonas stutzeri (R)-3-hydroxybutanoic acid
-
 ?
3.1.1.75 methyl (3R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoate + H2O
-
 Stutzerimonas stutzeri (R)-3-hydroxybutanoic acid + methyl-(R)-3-hydroxybutanoate + (R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoic acid
-
 ?
3.1.1.75 methyl (3R)-3-[[(3'R)-3'-methoxybutanoyl]oxy]butanoate + H2O
-
 Stutzerimonas stutzeri (R)-3-methoxybutanoic acid + methyl-(R)-3-hydroxybutanoate + methyl-[[(R)-3-methoxybutanoyl]oxy]butanoate
-
 ?
3.1.1.75 methyl (3R)-3-[[(3'R)-3'-[[(3''R)-3''-hydroxybutanoyl]oxy]butanoyl]oxy]butanoate + H2O
-
 Stutzerimonas stutzeri (R)-3-hydroxybutanoic acid + methyl-(R)-3-hydroxybutanoate + (R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoic acid + methyl (3R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoate + (R)-3-[[(3'R)-3'-[[(3''R)-3''-hydroxybutanoyl]oxy]butanoyl]oxy]butanoic acid
-
 ?
3.1.1.75 methyl (3R)-3-[[(3'R)-3'-[[(3''R)-3''-[[(3'''R)-3'''-hydroxybutanoyl]oxy]butanoyl]oxy]butanoyl]oxy]butanoate + H2O
-
 Stutzerimonas stutzeri (R)-3-hydroxybutanoic acid + methyl-(R)-3-hydroxybutanoate + (R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoic acid + methyl (3R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoate + (R)-3-[[(3'R)-3'-[[(3''R)-3''-hydroxybutanoyl]oxy]butanoyl]oxy]butanoic acid + methyl (3R)-3-[[(3'R)-3'-[[(3''R)-3''-[[(3'''R)-3'''-hydroxybutanoyl]oxy]butanoyl]oxy]butanoyl]oxy]butanoate
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O
-
 Stutzerimonas stutzeri poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?