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Literature summary extracted from

  • Ohura, T.; Kasuya, K.I.; Doi, Y.
    Cloning and characterization of the polyhydroxybutyrate depolymerase gene of Pseudomonas stutzeri and analysis of the function of substrate-binding domains (1999), Appl. Environ. Microbiol., 65, 189-197.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.75 phaZ PSTgene product, expression in Escherichia coli Pseudomonas stutzeri

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.75 additional information construction of several GST-fusion domains, analysis of substrate-binding domains Pseudomonas stutzeri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.75 extracellular
-
 Pseudomonas stutzeri
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O Pseudomonas stutzeri
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Pseudomonas stutzeri
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.75 cell culture
-
 Pseudomonas stutzeri
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 (R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoic acid + H2O
-
 Pseudomonas stutzeri (R)-3-hydroxybutanoic acid
-
 ?
3.1.1.75 methyl (3R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoate + H2O
-
 Pseudomonas stutzeri (R)-3-hydroxybutanoic acid + methyl-(R)-3-hydroxybutanoate + (R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoic acid
-
 ?
3.1.1.75 methyl (3R)-3-[[(3'R)-3'-methoxybutanoyl]oxy]butanoate + H2O
-
 Pseudomonas stutzeri (R)-3-methoxybutanoic acid + methyl-(R)-3-hydroxybutanoate + methyl-[[(R)-3-methoxybutanoyl]oxy]butanoate
-
 ?
3.1.1.75 methyl (3R)-3-[[(3'R)-3'-[[(3''R)-3''-hydroxybutanoyl]oxy]butanoyl]oxy]butanoate + H2O
-
 Pseudomonas stutzeri (R)-3-hydroxybutanoic acid + methyl-(R)-3-hydroxybutanoate + (R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoic acid + methyl (3R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoate + (R)-3-[[(3'R)-3'-[[(3''R)-3''-hydroxybutanoyl]oxy]butanoyl]oxy]butanoic acid
-
 ?
3.1.1.75 methyl (3R)-3-[[(3'R)-3'-[[(3''R)-3''-[[(3'''R)-3'''-hydroxybutanoyl]oxy]butanoyl]oxy]butanoyl]oxy]butanoate + H2O
-
 Pseudomonas stutzeri (R)-3-hydroxybutanoic acid + methyl-(R)-3-hydroxybutanoate + (R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoic acid + methyl (3R)-3-[[(3'R)-3'-hydroxybutanoyl]oxy]butanoate + (R)-3-[[(3'R)-3'-[[(3''R)-3''-hydroxybutanoyl]oxy]butanoyl]oxy]butanoic acid + methyl (3R)-3-[[(3'R)-3'-[[(3''R)-3''-[[(3'''R)-3'''-hydroxybutanoyl]oxy]butanoyl]oxy]butanoyl]oxy]butanoate
-
 ?
3.1.1.75 poly[(R)-3-hydroxybutanoate]n + H2O
-
 Pseudomonas stutzeri poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?