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Literature summary extracted from

  • Castanares, A.; Wood, T.M.
    Purification and characterization of a feruloyl/p-coumaroyl esterase from solid-state cultures of the aerobic fungus Penicillium pinophilum (1992), Biochem. Soc. Trans., 20, 275S.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.73 degradation the esterase capable to release phenolic acids from intact polymers, degradation may be of interest for industries wishing to effect the controlled degradation of plant cell walls Talaromyces pinophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.139
-
 methyl ferulate
-
 Talaromyces pinophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular
-
 Talaromyces pinophilus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 57000
-
 SDS-PAGE Talaromyces pinophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 feruloyl xylooligosaccharide + H2O Talaromyces pinophilus
-
 ferulic acid + xylooligosaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Schizophyllum commune
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O Talaromyces pinophilus
-
 ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyloligosaccharide cell wall fragments + H2O Schizophyllum commune
-
 ferulic acid + oligosaccharide cell wall fragments
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Schizophyllum commune
-
-
-
3.1.1.73 Talaromyces pinophilus
-
 CMI 87160 ii
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73
-
 Talaromyces pinophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 28.6
-
-
 Talaromyces pinophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 acetylxylan + H2O
-
 Talaromyces pinophilus acetic acid + ?
-
 ?
3.1.1.73 de-starched wheat bran + H2O
-
 Schizophyllum commune ferulic acid + ?
-
 ?
3.1.1.73 de-starched wheat bran + H2O
-
 Talaromyces pinophilus ferulic acid + ?
-
 ?
3.1.1.73 feruloyl xylooligosaccharide + H2O
-
 Talaromyces pinophilus ferulic acid + xylooligosaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Schizophyllum commune ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyl-polysaccharide + H2O
-
 Talaromyces pinophilus ferulate + polysaccharide
-
 ?
3.1.1.73 feruloyloligosaccharide cell wall fragments + H2O
-
 Schizophyllum commune ferulic acid + oligosaccharide cell wall fragments
-
 ?
3.1.1.73 p-coumaric acid methyl ester + H2O
-
 Talaromyces pinophilus p-coumaric acid + methanol
-
 ?
3.1.1.73 p-nitrophenyl acetate + H2O
-
 Talaromyces pinophilus p-nitrophenol + acetate
-
 ?
3.1.1.73 xylan polysaccharide + H2O
-
 Talaromyces pinophilus ferulic acid + p-coumaric acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 monomer 1 * 57000, SDS-PAGE Talaromyces pinophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 55
-
 methyl ferulate as substrate Talaromyces pinophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 37 55
-
 Talaromyces pinophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 37 60 purified enzyme is stable over the range 37-55°C for up to 8h, but loses activity rapidly at temperatures above 60°C Talaromyces pinophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 6
-
 methyl ferulate as substrate Talaromyces pinophilus