EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.83 | 1,2-Butanedione | - |
Pseudomonas fluorescens | |
1.1.1.83 | N-ethylmaleimide | 1 mM, 18% inactivation | Pseudomonas fluorescens | |
1.1.1.83 | p-chloromercuribenzoate | 0.2 mM, 10% inactivation | Pseudomonas fluorescens | |
1.1.1.84 | 1,2-Butanedione | - |
Pseudomonas fluorescens | |
1.1.1.84 | additional information | fairly stable against SH-group reactants | Pseudomonas fluorescens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.83 | 0.49 | - |
NAD+ | - |
Pseudomonas fluorescens | |
1.1.1.83 | 2.2 | - |
D-malate | - |
Pseudomonas fluorescens | |
1.1.1.84 | 0.15 | 0.2 | 3,3-dimethyl-D-malate | - |
Pseudomonas fluorescens | |
1.1.1.84 | 0.27 | - |
NAD+ | - |
Pseudomonas fluorescens | |
1.1.1.84 | 8.9 | 11 | D-malate | - |
Pseudomonas fluorescens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.83 | 34000 | - |
4 * 34000, SDS-PAGE | Pseudomonas fluorescens |
1.1.1.83 | 140000 | - |
gel filtration | Pseudomonas fluorescens |
1.1.1.84 | 34000 | - |
4 * 34000, SDS-PAGE | Pseudomonas fluorescens |
1.1.1.84 | 142000 | - |
gel filtration | Pseudomonas fluorescens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.84 | (R)-3,3-dimethylmalate + NAD+ | Pseudomonas fluorescens | - |
3-methyl-2-oxobutanoate + CO2 + NADH | - |
? | |
1.1.1.84 | (R)-3,3-dimethylmalate + NAD+ | Pseudomonas fluorescens UK-1 | - |
3-methyl-2-oxobutanoate + CO2 + NADH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.83 | Pseudomonas fluorescens | - |
UK-1 | - |
1.1.1.83 | Pseudomonas fluorescens UK-1 | - |
UK-1 | - |
1.1.1.84 | Pseudomonas fluorescens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.83 | - |
Pseudomonas fluorescens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.83 | 13.02 | - |
- |
Pseudomonas fluorescens |
1.1.1.84 | 12.54 | - |
- |
Pseudomonas fluorescens |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.83 | -70°C, pH 7.2, 1 mM EDTA | Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.83 | (R)-malate + NAD+ | - |
Pseudomonas fluorescens | pyruvate + CO2 + NADH | - |
? | |
1.1.1.83 | (R)-malate + NAD+ | - |
Pseudomonas fluorescens UK-1 | pyruvate + CO2 + NADH | - |
? | |
1.1.1.84 | (R)-3,3-dimethylmalate + NAD+ | - |
Pseudomonas fluorescens | 3-methyl-2-oxobutanoate + CO2 + NADH | - |
? | |
1.1.1.84 | (R)-3,3-dimethylmalate + NAD+ | - |
Pseudomonas fluorescens UK-1 | 3-methyl-2-oxobutanoate + CO2 + NADH | - |
? | |
1.1.1.84 | D-malate + NAD+ | - |
Pseudomonas fluorescens | pyruvate + CO2 + NADH | - |
? | |
1.1.1.84 | D-malate + NAD+ | - |
Pseudomonas fluorescens UK-1 | pyruvate + CO2 + NADH | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.83 | tetramer | 4 * 34000, SDS-PAGE | Pseudomonas fluorescens |
1.1.1.84 | tetramer | 4 * 34000, SDS-PAGE | Pseudomonas fluorescens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.83 | additional information | - |
NAD+ provides better protection against inactivation than D-malate or beta,beta-dimethyl-DL-malate | Pseudomonas fluorescens |
1.1.1.83 | 65 | - |
2 min, 64% loss of activity | Pseudomonas fluorescens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.83 | 8.4 | - |
- |
Pseudomonas fluorescens |
1.1.1.84 | 8.4 | - |
substrates 3,3-dimethylmalate + NAD+ | Pseudomonas fluorescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.83 | NAD+ | - |
Pseudomonas fluorescens | |
1.1.1.84 | NAD+ | - |
Pseudomonas fluorescens |