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Literature summary extracted from

  • Lähdesmäki, M.; Mäntsälä, P.
    Comparison of D-malate and beta, beta-dimethylmalate dehydrogenases from Pseudomonas fluorescens UK-1 (1980), Biochim. Biophys. Acta, 613, 266-274.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.83 1,2-Butanedione
-
Pseudomonas fluorescens
1.1.1.83 N-ethylmaleimide 1 mM, 18% inactivation Pseudomonas fluorescens
1.1.1.83 p-chloromercuribenzoate 0.2 mM, 10% inactivation Pseudomonas fluorescens
1.1.1.84 1,2-Butanedione
-
Pseudomonas fluorescens
1.1.1.84 additional information fairly stable against SH-group reactants Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.83 0.49
-
NAD+
-
Pseudomonas fluorescens
1.1.1.83 2.2
-
D-malate
-
Pseudomonas fluorescens
1.1.1.84 0.15 0.2 3,3-dimethyl-D-malate
-
Pseudomonas fluorescens
1.1.1.84 0.27
-
NAD+
-
Pseudomonas fluorescens
1.1.1.84 8.9 11 D-malate
-
Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.83 34000
-
4 * 34000, SDS-PAGE Pseudomonas fluorescens
1.1.1.83 140000
-
gel filtration Pseudomonas fluorescens
1.1.1.84 34000
-
4 * 34000, SDS-PAGE Pseudomonas fluorescens
1.1.1.84 142000
-
gel filtration Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.84 (R)-3,3-dimethylmalate + NAD+ Pseudomonas fluorescens
-
3-methyl-2-oxobutanoate + CO2 + NADH
-
?
1.1.1.84 (R)-3,3-dimethylmalate + NAD+ Pseudomonas fluorescens UK-1
-
3-methyl-2-oxobutanoate + CO2 + NADH
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.83 Pseudomonas fluorescens
-
UK-1
-
1.1.1.83 Pseudomonas fluorescens UK-1
-
UK-1
-
1.1.1.84 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.83
-
Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.83 13.02
-
-
Pseudomonas fluorescens
1.1.1.84 12.54
-
-
Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
1.1.1.83 -70°C, pH 7.2, 1 mM EDTA Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.83 (R)-malate + NAD+
-
Pseudomonas fluorescens pyruvate + CO2 + NADH
-
?
1.1.1.83 (R)-malate + NAD+
-
Pseudomonas fluorescens UK-1 pyruvate + CO2 + NADH
-
?
1.1.1.84 (R)-3,3-dimethylmalate + NAD+
-
Pseudomonas fluorescens 3-methyl-2-oxobutanoate + CO2 + NADH
-
?
1.1.1.84 (R)-3,3-dimethylmalate + NAD+
-
Pseudomonas fluorescens UK-1 3-methyl-2-oxobutanoate + CO2 + NADH
-
?
1.1.1.84 D-malate + NAD+
-
Pseudomonas fluorescens pyruvate + CO2 + NADH
-
?
1.1.1.84 D-malate + NAD+
-
Pseudomonas fluorescens UK-1 pyruvate + CO2 + NADH
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.83 tetramer 4 * 34000, SDS-PAGE Pseudomonas fluorescens
1.1.1.84 tetramer 4 * 34000, SDS-PAGE Pseudomonas fluorescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.83 additional information
-
NAD+ provides better protection against inactivation than D-malate or beta,beta-dimethyl-DL-malate Pseudomonas fluorescens
1.1.1.83 65
-
2 min, 64% loss of activity Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.83 8.4
-
-
Pseudomonas fluorescens
1.1.1.84 8.4
-
substrates 3,3-dimethylmalate + NAD+ Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.83 NAD+
-
Pseudomonas fluorescens
1.1.1.84 NAD+
-
Pseudomonas fluorescens