EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.262 | overexpression in Escherichia coli | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.262 | EDTA | inhibition at 1 mM, fully restored by addition of 1 mM Mn2+, Co2+, Mg2+ or Ca2+, partially restored by 1 mM Ni2+ or Zn2+ | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.262 | 4-(phosphohydroxy)-L-threonine + NAD+ | Escherichia coli | involved in pyridoxal 5'-phosphate formation | 3-phosphohydroxy-1aminoacetone + CO2 + NADH + H+ | further condensation with 1-deoxy-D-xylulose-5-phosphate to form pyridoxal 5'-phosphate | r | |
2.6.99.2 | 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate | Escherichia coli | the enzyme is involved in biosynthesis of vitamin B6 | pyridoxine 5'-phosphate + phosphate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.262 | Escherichia coli | - |
- |
- |
2.6.99.2 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.262 | to homogeneity, recombinant enzyme | Escherichia coli |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.6.99.2 | 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = pyridoxine 5'-phosphate + phosphate + 2 H2O | reaction mechanism, the enzyme shows phosphomutase activity | Escherichia coli |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.262 | additional information | pdxA gene product | Escherichia coli | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.262 | 4-(phosphohydroxy)-L-threonine + NAD+ | involved in pyridoxal 5'-phosphate formation | Escherichia coli | 3-phosphohydroxy-1aminoacetone + CO2 + NADH + H+ | further condensation with 1-deoxy-D-xylulose-5-phosphate to form pyridoxal 5'-phosphate | r | |
2.6.99.2 | 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate | the enzyme is involved in biosynthesis of vitamin B6 | Escherichia coli | pyridoxine 5'-phosphate + phosphate + H2O | - |
? | |
2.6.99.2 | 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate | the enzyme shows phosphomutase activity transfering the 5-phosphoryl group of 1-deoxy-D-xylose 5-phosphate to the 4-hydroxyl at some point in the reaction, the phosphate group of the substrate is required for activity, reaction mechanism, overview | Escherichia coli | pyridoxine 5'-phosphate + phosphate + H2O | i.e. pyridoxol phosphate | ? | |
2.6.99.2 | additional information | free alcohol deoxyxylulose is no substrate | Escherichia coli | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.99.2 | PdxJ | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.99.2 | 37 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.99.2 | 7.5 | - |
assay at | Escherichia coli |