Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Laber, B.; Maurer, W.; Scharf, S.; Stepusin, K.; Schmidt, F.S.
    Vitamin B6 biosynthesis: formation of pyridoxine 5'-phosphate from 4-(phosphohydroxy)-L-threonine and 1-deoxy-D-xylulose-5-phosphate by PdxA and PdxJ protein (1999), FEBS Lett., 449, 45-48.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.262 overexpression in Escherichia coli Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.262 4-(phosphohydroxy)-L-threonine + NAD+ Escherichia coli involved in pyridoxal 5'-phosphate formation 2-amino-3-oxo-4-phosphohydroxybutyrate + NADH further condensation with 1-deoxy-D-xylulose-5-phosphate to form pyridoxal 5'-phosphate r
2.6.99.2 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate Escherichia coli last step in the pyridoxine 5'-phosphate biosynthetic pathway, overview pyridoxine 5'-phosphate + phosphate + H2O
-
 ?
2.6.99.2 additional information Escherichia coli no activity with 1-deoxy-D-xylose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.262 Escherichia coli
-
-
-
2.6.99.2 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.262 to homogeneity, recombinant enzyme Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.99.2 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = pyridoxine 5'-phosphate + phosphate + 2 H2O reaction mechanism Escherichia coli
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.262 additional information pdxA gene product Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.262 4-(phosphohydroxy)-L-threonine + NAD+ involved in pyridoxal 5'-phosphate formation Escherichia coli 2-amino-3-oxo-4-phosphohydroxybutyrate + NADH further condensation with 1-deoxy-D-xylulose-5-phosphate to form pyridoxal 5'-phosphate r
2.6.99.2 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate
-
 Escherichia coli pyridoxine 5'-phosphate + phosphate + H2O
-
 ?
2.6.99.2 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate last step in the pyridoxine 5'-phosphate biosynthetic pathway, overview Escherichia coli pyridoxine 5'-phosphate + phosphate + H2O
-
 ?
2.6.99.2 additional information no activity with 1-deoxy-D-xylose Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.99.2 PdxJ
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.99.2 37
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.262 1.4
-
 4-(phosphohydroxy)-L-threonine
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.99.2 7.8
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.262 NAD+
-
 Escherichia coli