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Literature summary extracted from

  • Okada, H.; Yohda, M.; Giga-Hama, Y.; Ueno, Y.; Ohdo, S.; Kumagai, H.
    Distribution and purification of asparate racemase in lactic acid bacteria (1991), Biochim. Biophys. Acta, 1078, 377-382.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.1.1.13 SH-protecting reagents e.g., 2-mercaptoethanol and dithiothreitol increase activity 2fold Streptococcus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.13 28000
-
 2 * 28000, SDS-PAGE Streptococcus thermophilus
5.1.1.13 60000
-
 gel filtration Streptococcus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.13 Enterococcus faecalis
-
 strain ATCC 9790, strain IAM 10065, no activity in strain IAM 10067
-
5.1.1.13 Enterococcus faecium
-
-
-
5.1.1.13 Lacticaseibacillus casei
-
-
-
5.1.1.13 Lactobacillus delbrueckii
-
-
-
5.1.1.13 Lactobacillus delbrueckii subsp. bulgaricus
-
-
-
5.1.1.13 Lactococcus lactis
-
-
-
5.1.1.13 Levilactobacillus brevis
-
-
-
5.1.1.13 no activity in Bifidobacterium bifidum
-
-
-
5.1.1.13 no activity in Leuconostoc mesenteroides
-
-
-
5.1.1.13 no activity in Pediococcus pentosaceus
-
-
-
5.1.1.13 Streptococcus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.13
-
 Streptococcus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.13 26.8
-
-
 Streptococcus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.13 L-Ala no racemase activity Streptococcus thermophilus D-Ala
-
 ?
5.1.1.13 L-Asp
-
 Lactococcus lactis D-Asp
-
 ?
5.1.1.13 L-Asp
-
 Enterococcus faecalis D-Asp
-
 ?
5.1.1.13 L-Asp
-
 Lacticaseibacillus casei D-Asp
-
 ?
5.1.1.13 L-Asp
-
 Enterococcus faecium D-Asp
-
 ?
5.1.1.13 L-Asp
-
 Streptococcus thermophilus D-Asp
-
 ?
5.1.1.13 L-Asp
-
 Levilactobacillus brevis D-Asp
-
 ?
5.1.1.13 L-Asp
-
 Lactobacillus delbrueckii subsp. bulgaricus D-Asp
-
 ?
5.1.1.13 L-Asp
-
 Lactobacillus delbrueckii D-Asp
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.1.13 dimer 2 * 28000, SDS-PAGE Streptococcus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.13 30
-
-
 Lactococcus lactis
5.1.1.13 37
-
-
 Streptococcus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.1.13 37
-
 1 h, 50 mM potassium phosphate buffer, stable Streptococcus thermophilus
5.1.1.13 37
-
 2 h, complete inactivation Lactococcus lactis
5.1.1.13 50
-
 1 h, about 60% loss of activity, pH 7.2 Streptococcus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.13 7.2
-
-
 Streptococcus thermophilus
5.1.1.13 8
-
-
 Streptococcus thermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.1.13 5.5 7.5 stable Streptococcus thermophilus