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Literature summary extracted from

  • Reinberg, D.; Arenas, J.; Hurwitz, J.
    The enzymatic conversion of 3'-phosphate terminated RNA chains to 2',3'-cyclic phosphate derivatives (1985), J. Biol. Chem., 260, 6088-6097.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.4 NEM
-
 Homo sapiens
6.5.1.4 SDS
-
 Homo sapiens
6.5.1.4 Sodium phosphate
-
 Homo sapiens
6.5.1.4 Vanadyl-ribonucleoside complex
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.4 0.002
-
 ATP
-
 Homo sapiens
6.5.1.4 0.006
-
 ATPgammaS
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.4 Mg2+ required Homo sapiens
6.5.1.4 Mg2+ Km: 2.5 mM Homo sapiens
6.5.1.4 Mn2+ cannot replace Mg2+ in activation Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.4 38000 40000 glycerol density gradient sedimentation, gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.4
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.5.1.4 HeLa cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.5.1.4 0.021
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
6.5.1.4 -70°C, stable for at least 3 months Homo sapiens
6.5.1.4 4°C, 50% loss of activity after 3 days Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.4 ATP + RNA 3'-terminal-phosphate ir Homo sapiens AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATP + RNA 3'-terminal-phosphate (Up)10Gp is cyclized at a faster rate than tRNAApGp or AUGp Homo sapiens AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 ATPgammaS + RNA 3'-terminal-phosphate ir Homo sapiens AMP + thiodiphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
6.5.1.4 additional information ATP/diphosphate exchange reaction not detected Homo sapiens ?
-
 ?