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Literature summary extracted from

  • Silber, R.; Malathi, V.G.; Hurwitz, J.
    Purification and properties of bacteriophage T4-induced RNA ligase (1972), Proc. Natl. Acad. Sci. USA, 69, 3009-3013.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.3 0.0002
-
 ATP
-
 Tequatrovirus T4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.3 Mg2+ required Tequatrovirus T4
6.5.1.3 Mg2+ Km: 0.4 mM, inhibition above 10 mM Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.3 52000
-
 gel filtration Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.3 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.3
-
 Tequatrovirus T4

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.5.1.3 0.0383
-
 5'-phosphate terminus rendered resistant to phosphatase Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m converts linear molecules of polyriboadenylate to circular form by the intramolecular covalent linkage of the 5'-phosphate end to the 3'-hydroxyl terminus Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
 ?
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m the best substrate is [5'-P]polyriboadenylate Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
 ?
6.5.1.3 dATP + (ribonucleotide)n + (ribonucleotide)m at 20% the activity relative to ATP Tequatrovirus T4 dAMP + phosphate + (ribonucleotide)n+m
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.3 7.5 8.2
-
 Tequatrovirus T4