BRENDA - Enzyme Database

Purification and characterization of Chlamydomonas reinhardtii chloroplast glutamyl-tRNA synthetase, a natural misacylating enzyme

Chen, M.W.; Jahn, D.; Schon, A.; O'Neill, G.P.O.; S÷ll, D.; J. Biol. Chem. 265, 4054-4057 (1990)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.1
-
ATP
-
Chlamydomonas reinhardtii
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.1.1.17
chloroplast
-
Chlamydomonas reinhardtii
9507
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
62000
-
sucrose density gradient centrifugation HPLC gel filtration
Chlamydomonas reinhardtii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Chlamydomonas reinhardtii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.1.1.17
-
Chlamydomonas reinhardtii
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
6.1.1.17
0.008
-
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + glutamate + chloroplastic tRNAGln
misacylation
199
Chlamydomonas reinhardtii
AMP + diphosphate + gluamyl-tRNAGln
-
-
-
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
glutamylate E. coli tRNAGluF, not cytoplasmic tRNAGlu from yeast and barley
199
Chlamydomonas reinhardtii
AMP + diphosphate + L-glutamyl-tRNAGlu
-
199
Chlamydomonas reinhardtii
-
Subunits
EC Number
Subunits
Commentary
Organism
6.1.1.17
monomer
1 * 62000, SDS-PAGE
Chlamydomonas reinhardtii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.1
-
ATP
-
Chlamydomonas reinhardtii
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.1.1.17
chloroplast
-
Chlamydomonas reinhardtii
9507
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
62000
-
sucrose density gradient centrifugation HPLC gel filtration
Chlamydomonas reinhardtii
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.17
-
Chlamydomonas reinhardtii
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
6.1.1.17
0.008
-
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + glutamate + chloroplastic tRNAGln
misacylation
199
Chlamydomonas reinhardtii
AMP + diphosphate + gluamyl-tRNAGln
-
-
-
-
6.1.1.17
ATP + L-glutamate + tRNAGlu
glutamylate E. coli tRNAGluF, not cytoplasmic tRNAGlu from yeast and barley
199
Chlamydomonas reinhardtii
AMP + diphosphate + L-glutamyl-tRNAGlu
-
199
Chlamydomonas reinhardtii
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.1.1.17
monomer
1 * 62000, SDS-PAGE
Chlamydomonas reinhardtii