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Literature summary extracted from

  • Chen, M.W.; Jahn, D.; Schon, A.; O'Neill, G.P.O.; Söll, D.
    Purification and characterization of Chlamydomonas reinhardtii chloroplast glutamyl-tRNA synthetase, a natural misacylating enzyme (1990), J. Biol. Chem., 265, 4054-4057.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.17 0.1
-
 ATP
-
 Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.17 chloroplast
-
 Chlamydomonas reinhardtii 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.17 62000
-
 sucrose density gradient centrifugation HPLC gel filtration Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.17 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.17
-
 Chlamydomonas reinhardtii

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.17 0.008
-
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.17 ATP + glutamate + chloroplastic tRNAGln misacylation Chlamydomonas reinhardtii AMP + diphosphate + gluamyl-tRNAGln
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu glutamylate E. coli tRNAGluF, not cytoplasmic tRNAGlu from yeast and barley Chlamydomonas reinhardtii AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.17 monomer 1 * 62000, SDS-PAGE Chlamydomonas reinhardtii