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Literature summary extracted from

  • Pick, L.; Hurwitz, J.
    Purification of wheat germ RNA ligase. I. Characterization of a ligase-associated 5'-hydroxyl polynucleotide kinase activity (1986), J. Biol. Chem., 261, 6684-6693.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.5.1.3 activity is unaffected by at least 3 freeze-thaw cycles Triticum aestivum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.3 Mg2+ required Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.3 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.3 copurifies with 5'-hydroxyl polynucleotide kinase Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.5.1.3 germ
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.5.1.3 additional information
-
-
 Triticum aestivum

Storage Stability

EC Number Storage Stability Organism
6.5.1.3 -70°C, stable for at least 1 year Triticum aestivum
6.5.1.3 4°C, 32% loss of activity after 2 weeks Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m
-
 Triticum aestivum AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.5.1.3 30
-
 stable for at least 2 h Triticum aestivum
6.5.1.3 56
-
 2 min, complete inactivation Triticum aestivum