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Literature summary extracted from
- Characterization of DNA ligase from the fungus Coprinus cinereus (1996), Eur. J. Biochem., 237, 691-697.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.5.1.1 | p-chloromercuribenzoate | - |
Coprinopsis cinerea |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 6.5.1.1 | 0.00009 | - |
(dT)20 | hybridized with (dA) | Coprinopsis cinerea | |
| 6.5.1.1 | 0.0015 | - |
ATP | - |
Coprinopsis cinerea | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.5.1.1 | Mg2+ | required | Coprinopsis cinerea | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 6.5.1.1 | 88000 | - |
1 * 88000, SDS-PAGE | Coprinopsis cinerea |
| 6.5.1.1 | 90000 | - |
gel filtration | Coprinopsis cinerea |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.5.1.1 | Coprinopsis cinerea | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.5.1.1 | - |
Coprinopsis cinerea |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 6.5.1.1 | mycelium | mitotic cell | Coprinopsis cinerea | - |
| 6.5.1.1 | pachytene cell | - |
Coprinopsis cinerea | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 6.5.1.1 | additional information | - |
- |
Coprinopsis cinerea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.5.1.1 | ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | capable of joining (dT)20*(rA)n and (rA)12-18*(dT)n as well as (dT)20*(dA)n, ligation of blunt-ended DNA in the presence of polyethylene glycol 6000 | Coprinopsis cinerea | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
| 6.5.1.1 | ATP + (dT)20 | - |
Coprinopsis cinerea | AMP + diphosphate + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 6.5.1.1 | monomer | 1 * 88000, SDS-PAGE | Coprinopsis cinerea |
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