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Literature summary extracted from

  • Wood, P.H.; Sundaram, T.K.; Blackburn, N.J.; Marsh, A.E.
    Isolation and characterization of pyruvate carboxylase from the extremely thermophilic Bacillus caldotenax (1983), Biochem. Soc. Trans., 11, 741-742.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.4.1.1 acetyl-CoA allosteric activator. Concentrations eliciting maximal activity are 0.013 mM, 0.042 mM and 0.084 mM at assay temperatures of 45°C, 55°C, and 65°C respectively [Bacillus] caldotenax

General Stability

EC Number General Stability Organism
6.4.1.1 stable to exposure to 6 M urea for up to 2.5 h at 20°C [Bacillus] caldotenax

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.1 Avidin strong [Bacillus] caldotenax
6.4.1.1 L-Asp
-
 [Bacillus] caldotenax

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.1 Zinc contains 4 atoms of zinc per molecule [Bacillus] caldotenax

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.1 136000
-
 x * 136000 [Bacillus] caldotenax
6.4.1.1 537000
-
-
 [Bacillus] caldotenax

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.4.1.1 ATP + pyruvate + HCO3- [Bacillus] caldotenax meets anaplerotic and/or biosynthetic needs in metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.1 [Bacillus] caldotenax
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.1
-
 [Bacillus] caldotenax

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.1 3.7
-
-
 [Bacillus] caldotenax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.1 ATP + pyruvate + HCO3-
-
 [Bacillus] caldotenax ADP + phosphate + oxaloacetate
-
 ?
6.4.1.1 ATP + pyruvate + HCO3- meets anaplerotic and/or biosynthetic needs in metabolism [Bacillus] caldotenax ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.1 ? x * 136000 [Bacillus] caldotenax

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.4.1.1 60
-
 pH 7, half-life: 1 h [Bacillus] caldotenax

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.1 biotin enzyme contains biotin [Bacillus] caldotenax
6.4.1.1 biotin enzyme contains covalently bound D-biotin [Bacillus] caldotenax