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Literature summary extracted from

  • Hirabayashi, Y.; Kimura, M.; Matsumoto, M.; Yamamoto, K.; Kadowaki, S.; Tochikura, T.
    A novel glycosphingolipid hydrolyzing enzyme, glycosphingolipid ceramide deacylase, which cleaves the linkage between the fatty acid and sphingosine base in glycosphingolipids (1988), J. Biochem., 103, 1-4.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.23 Ba2+
-
 Nocardia sp.
3.5.1.23 Mg2+
-
 Nocardia sp.
3.5.1.23 sodium taurodeoxycholate 1 mg/ml Nocardia sp.
3.5.1.69 sodium taurodeoxycholate 1.0 mg/ml, slight activation Nocardia sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.23 Cu2+ at 10 mM Nocardia sp.
3.5.1.23 EDTA at 10 mM Nocardia sp.
3.5.1.23 Hg2+ at 10 mM Nocardia sp.
3.5.1.23 Mn2+ at 10 mM Nocardia sp.
3.5.1.23 Sn2+ at 10 mM Nocardia sp.
3.5.1.23 Zn2+ at 10 mM Nocardia sp.
3.5.1.69 Cu2+ 10 mM, strong Nocardia sp.
3.5.1.69 EDTA 10 mM, 30% inhibition Nocardia sp.
3.5.1.69 Hg2+ 10 mM, strong Nocardia sp.
3.5.1.69 Mn2+ 10 mM, strong Nocardia sp.
3.5.1.69 Sn2+ 10 mM, strong Nocardia sp.
3.5.1.69 Zn2+ 10 mM, strong Nocardia sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 membrane
-
 Nocardia sp. 16020
-
3.5.1.69 membrane tightly bound to Nocardia sp. 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.69 Ba2+ 10 mM, slight activation Nocardia sp.
3.5.1.69 Mg2+ 10 mM, slight activation Nocardia sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Nocardia sp.
-
-
-
3.5.1.69 Nocardia sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 ganglioside + H2O GM1, GM2, GM3 Nocardia sp. lyso-ganglioside + fatty acid corresponding to ganglioside type ?
3.5.1.69 ganglioside asialo-GM1 + H2O
-
 Nocardia sp. ?
-
 ?
3.5.1.69 ganglioside asialo-GM2 + H2O
-
 Nocardia sp. ?
-
 ?
3.5.1.69 ganglioside Gb3-ceramide + H2O
-
 Nocardia sp. ?
-
 ?
3.5.1.69 ganglioside GD1a + H2O
-
 Nocardia sp. fatty acid + lyso-GD1a-ganglioside
-
 ?
3.5.1.69 ganglioside GM1 + H2O
-
 Nocardia sp. octadecanoic acid + lyso-GM1-ganglioside
-
 ?
3.5.1.69 ganglioside GM2 + H2O
-
 Nocardia sp. fatty acid + lyso-GM2-ganglioside
-
 ?
3.5.1.69 ganglioside GM3 + H2O
-
 Nocardia sp. fatty acid + lyso-GM3-ganglioside
-
 ?
3.5.1.69 additional information does not act on sphingolipids such as ceramide, Galbeta(1-1')ceramide, Glcbeta(1-1')ceramide, Galbeta(1-4)Glcbeta(1-1')ceramide Nocardia sp. ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.23 45
-
 stable below 45°C Nocardia sp.
3.5.1.69 45
-
 pH 7.2, 30 min, stable Nocardia sp.
3.5.1.69 55
-
 pH 7.2, 30 min, 50% loss of activity Nocardia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 5.8
-
-
 Nocardia sp.
3.5.1.69 5.8
-
-
 Nocardia sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.69 7.2
-
 45°C, 30 min, stable Nocardia sp.