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Literature summary extracted from

  • Kalvero Airas, R.; Hietanen, E.A.; Nurmikko, V.T.
    Purification and properties of pantothenase from Pseudomonas fluorescens (1976), Biochem. J., 157, 409-413.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.22 50000
-
 2 * 50000, SDS-PAGE Pseudomonas fluorescens
3.5.1.22 97400
-
 gel filtration Pseudomonas fluorescens
3.5.1.22 100000
-
 gel filtration Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.22 pantothenic acid + H2O Pseudomonas fluorescens
-
 ?
-
 ?
3.5.1.22 pantothenic acid + H2O Pseudomonas fluorescens P-2
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.22 Pseudomonas fluorescens
-
 P-2
-
3.5.1.22 Pseudomonas fluorescens
-
 biotype C
-
3.5.1.22 Pseudomonas fluorescens
-
 NCIB12017, earlier Pseudomonas fluorescens UK-1, isolated from sea water
-
3.5.1.22 Pseudomonas fluorescens P-2
-
 P-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.22
-
 Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.22 13.2
-
-
 Pseudomonas fluorescens
3.5.1.22 86.9
-
 strain P-2 Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
3.5.1.22 -20°C 20 mM potassium phosphate pH 7, enzyme is made accessible to substrates by freezing and thawing four times, decrease of activity 20% per round of freezing and thawing, frozen enzyme retains activity for at least 2 years Pseudomonas fluorescens
3.5.1.22 -20°C 35 mM potassium phosphate, pH 6.8 Pseudomonas fluorescens
3.5.1.22 2°C 5 mM K2SO4, 1 week, activity dimished to 50% Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.22 pantothenic acid + H2O
-
 Pseudomonas fluorescens beta-alanine + D-pantoyl lactone + H2O
-
 r
3.5.1.22 pantothenic acid + H2O
-
 Pseudomonas fluorescens P-2 beta-alanine + D-pantoyl lactone + H2O
-
 r
3.5.1.22 pantothenic acid + H2O
-
 Pseudomonas fluorescens ?
-
 ?
3.5.1.22 pantothenic acid + H2O
-
 Pseudomonas fluorescens P-2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.22 dimer 2 * 50000, SDS-PAGE Pseudomonas fluorescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.22 28 40 heat inactivation occurs above 28°C, inactivated at 40°C Pseudomonas fluorescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.22 5.5 10 below pH 5 activity rapidly decreases Pseudomonas fluorescens