Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Thoma, R.W.; Peterson, W.H.
    The enzymatic degradation of soluble bound biotin (1954), J. Biol. Chem., 210, 569-579.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.12 Mg2+ activator might be need to give full release of biotin, erratic effect Sus scrofa
3.5.1.12 Zn2+ activator might be need to give full release of biotin, erratic effect Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.12 biotin amide + H2O Gallus sp.
-
 ?
-
 ?
3.5.1.12 biotin amide + H2O Sus scrofa soluble bound biotin ?
-
 ?
3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O Gallus sp.
-
 ?
-
 ?
3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O Sus scrofa biocytin, soluble bound biotin ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.5.1.12 Ethanol no loss of enzyme occurs in 10% at 0°C, at 50% all activity lost Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.12 Gallus sp.
-
 chicken
-
3.5.1.12 no activity in Lactobacillus arabinosus
-
-
-
3.5.1.12 Sus scrofa
-
 pig
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.12 kidney
-
 Sus scrofa
-
3.5.1.12 liver
-
 Sus scrofa
-
3.5.1.12 pancreas
-
 Gallus sp.
-

Storage Stability

EC Number Storage Stability Organism
3.5.1.12 15°C enzyme solution with toluene, for a week or more without appreciable loss of activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.12 biotin amide + H2O
-
 Gallus sp. ?
-
 ?
3.5.1.12 biotin amide + H2O soluble bound biotin Sus scrofa ?
-
 ?
3.5.1.12 biotin methyl ester + H2O
-
 Sus scrofa ?
-
 ?
3.5.1.12 biotin methyl ester + H2O
-
 Gallus sp. ?
-
 ?
3.5.1.12 biotinyl-L-aspartate + H2O
-
 Sus scrofa biotin + L-aspartate
-
 ?
3.5.1.12 biotinyl-L-aspartate + H2O
-
 Gallus sp. biotin + L-aspartate
-
 ?
3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O
-
 Gallus sp. biotin + L-lysine
-
 ir
3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O biocytin, soluble bound biotin Sus scrofa biotin + L-lysine
-
 ir
3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O
-
 Gallus sp. ?
-
 ?
3.5.1.12 epsilon-N-biotinyl-L-lysine + H2O biocytin, soluble bound biotin Sus scrofa ?
-
 ?
3.5.1.12 N-biotinyl-beta-alanine + H2O
-
 Sus scrofa biotin + beta-alanine
-
 ?
3.5.1.12 N-biotinyl-beta-alanine + H2O
-
 Gallus sp. biotin + beta-alanine
-
 ?
3.5.1.12 p-(N-biotinylamino)benzoic acid + H2O
-
 Sus scrofa biotin + p-aminobenzoic acid
-
 ir
3.5.1.12 p-(N-biotinylamino)benzoic acid + H2O
-
 Gallus sp. biotin + p-aminobenzoic acid
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.12 38
-
-
 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.12 6.8 7
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.12 5 8.5
-
 Sus scrofa