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Literature summary extracted from
- Structural and kinetic properties of chymotrypsin from atlantic cod (Gadus morhua). Comparison with bovine chymotrypsin (1991), Comp. Biochem. Physiol. B, 99B, 327-335.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.1 | 2-mercaptoethanol | - |
Gadus morhua |  |
| 3.4.21.1 | chymostatin | - |
Bos taurus | |
| 3.4.21.1 | chymostatin | - |
Gadus morhua | |
| 3.4.21.1 | Cys | - |
Gadus morhua | |
| 3.4.21.1 | p-tosyl-L-phenylalanine chloromethane | - |
Gadus morhua | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.1 | 0.08 | - |
N-benzoyl-Tyr p-nitroanilide | enzyme form A and enzyme form B, at 25°C | Gadus morhua | |
| 3.4.21.1 | 0.09 | - |
N-benzoyl-Tyr p-nitroanilide | enzyme form A, at 10°C | Gadus morhua | |
| 3.4.21.1 | 0.1 | - |
N-benzoyl-L-Tyr ethyl ester | enzyme form B, at 35°C | Gadus morhua | |
| 3.4.21.1 | 0.11 | - |
N-benzoyl-Tyr p-nitroanilide | enzyme form B, at 35°C | Gadus morhua | |
| 3.4.21.1 | 0.11 | - |
N-benzoyl-Tyr p-nitroanilide | at 10°C | Bos taurus | |
| 3.4.21.1 | 0.11 | - |
N-benzoyl-Tyr p-nitroanilide | enzyme form B, at 10°C | Gadus morhua | |
| 3.4.21.1 | 0.12 | - |
N-benzoyl-L-Tyr ethyl ester | enzyme form A, at 10°C | Gadus morhua | |
| 3.4.21.1 | 0.14 | - |
N-benzoyl-Tyr p-nitroanilide | at 25°C | Bos taurus | |
| 3.4.21.1 | 0.14 | - |
N-benzoyl-Tyr p-nitroanilide | enzyme form A, at 35°C | Gadus morhua | |
| 3.4.21.1 | 0.14 | - |
N-benzoyl-L-Tyr ethyl ester | enzyme form A, at 25°C | Gadus morhua | |
| 3.4.21.1 | 0.17 | - |
N-benzoyl-L-Tyr ethyl ester | enzyme form A, at 35°C | Gadus morhua | |
| 3.4.21.1 | 0.2 | - |
N-benzoyl-L-Tyr ethyl ester | enzyme form B, at 25°C or 10°C | Gadus morhua | |
| 3.4.21.1 | 0.21 | - |
N-benzoyl-Tyr p-nitroanilide | at 35°C | Bos taurus | |
| 3.4.21.1 | 0.24 | - |
N-benzoyl-L-Tyr ethyl ester | at 35°C | Bos taurus | |
| 3.4.21.1 | 0.27 | - |
N-benzoyl-L-Tyr ethyl ester | at 25°C or at 10°C | Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.1 | 26000 | - |
gel filtration | Gadus morhua |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.1 | Bos taurus | - |
- |
- |
| 3.4.21.1 | Gadus morhua | - |
2 enzyme forms: A and B | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.1 | 154 | - |
enzyme form A | Gadus morhua |
| 3.4.21.1 | 178 | - |
enzyme form B | Gadus morhua |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.1 | N-benzoyl-L-Tyr ethyl ester + H2O | - |
Bos taurus | N-benzoyl-L-Tyr + ethanol | - |
? | |
| 3.4.21.1 | N-benzoyl-L-Tyr ethyl ester + H2O | - |
Gadus morhua | N-benzoyl-L-Tyr + ethanol | - |
? | |
| 3.4.21.1 | N-benzoyl-Tyr p-nitroanilide + H2O | - |
Bos taurus | N-benzoyl-Tyr + p-nitroaniline | - |
? | |
| 3.4.21.1 | N-benzoyl-Tyr p-nitroanilide + H2O | - |
Gadus morhua | N-benzoyl-Tyr + p-nitroaniline | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.1 | monomer | 1 * 26000, SDS-PAGE | Gadus morhua |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.1 | additional information | - |
additional information | influence of dimethylsulfoxide on the turnover-number | Bos taurus | |
| 3.4.21.1 | 0.05 | - |
N-benzoyl-Tyr p-nitroanilide | at pH 10°C | Bos taurus | |
| 3.4.21.1 | 0.1 | - |
N-benzoyl-Tyr p-nitroanilide | enzyme form A and enzyme form B, at pH 10°C | Gadus morhua | |
| 3.4.21.1 | 0.3 | - |
N-benzoyl-Tyr p-nitroanilide | at 25°C | Bos taurus | |
| 3.4.21.1 | 0.41 | - |
N-benzoyl-Tyr p-nitroanilide | at 35°C | Bos taurus | |
| 3.4.21.1 | 0.41 | - |
N-benzoyl-Tyr p-nitroanilide | enzyme form A, 25°C | Gadus morhua | |
| 3.4.21.1 | 0.42 | - |
N-benzoyl-Tyr p-nitroanilide | enzyme form B, at 25°C | Gadus morhua | |
| 3.4.21.1 | 0.72 | - |
N-benzoyl-Tyr p-nitroanilide | enzyme form B, at 25°C | Gadus morhua | |
| 3.4.21.1 | 0.82 | - |
N-benzoyl-Tyr p-nitroanilide | enzyme form A, at 35°C | Gadus morhua | |
| 3.4.21.1 | 43 | - |
N-benzoyl-L-Tyr ethyl ester | at 10°C | Bos taurus | |
| 3.4.21.1 | 100 | - |
N-benzoyl-L-Tyr ethyl ester | at 35°C | Bos taurus | |
| 3.4.21.1 | 117 | - |
N-benzoyl-L-Tyr ethyl ester | enzyme form A, at 10°C | Gadus morhua | |
| 3.4.21.1 | 120 | - |
N-benzoyl-L-Tyr ethyl ester | enzyme form B, at 10°C | Gadus morhua | |
| 3.4.21.1 | 142 | - |
N-benzoyl-L-Tyr ethyl ester | at 35°C | Bos taurus | |
| 3.4.21.1 | 207 | - |
N-benzoyl-L-Tyr ethyl ester | enzyme form A, at 25°C | Gadus morhua | |
| 3.4.21.1 | 214 | - |
N-benzoyl-L-Tyr ethyl ester | enzyme form B, at 32°C | Gadus morhua | |
| 3.4.21.1 | 276 | - |
N-benzoyl-L-Tyr ethyl ester | enzyme form B, at 35°C | Gadus morhua | |
| 3.4.21.1 | 329 | - |
N-benzoyl-L-Tyr ethyl ester | enzyme form A, at 35°C | Gadus morhua | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.1 | 7.8 | - |
hydrolysis of N-benzoyl-L-Tyr ethyl ester | Bos taurus |
| 3.4.21.1 | 7.8 | - |
hydrolysis of N-benzoyl-L-Tyr ethyl ester | Gadus morhua |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.1 | 6 | 9.3 | pH 6.0: about 35% of maximal activity, pH 9.3: 40% of maximal activity | Bos taurus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.1 | 2 | - |
5°C, about 55% loss of activity after 60 min | Gadus morhua |
| 3.4.21.1 | 3 | - |
5°C, about 65% loss of activity after 60 min | Gadus morhua |
| 3.4.21.1 | 4 | - |
5°C, about 30% loss of activity after 60 min | Gadus morhua |
| 3.4.21.1 | 5 | 12 | 5°C, stable for 60 min | Gadus morhua |
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