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Literature summary extracted from

  • Karran, P.; Lindahl, T.
    Enzymatic excision of free hypoxanthine from polydeoxynucleotides and DNA containing deoxyinosine monophosphate residues (1978), J. Biol. Chem., 253, 5877-5879.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.2.15 relatively sensitive to inhibition by neutral salts Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.15 NaCl 70% inhibited by addition of 0.1 M NaCl Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.15 30000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.15 Escherichia coli
-
 K-12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.15 partially Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.2.15 hypoxanthine-containing DNA + H2O = hypoxanthine + DNA containing an abasic site specific enzymatic cleavage of the glycosyl bond Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.15 DNA + H2O more efficient on double-stranded than of single-stranded substrates, activity 10-15 times higher, removal of nonconventional residues from DNA Escherichia coli hypoxanthine + ?
-
 ?
3.2.2.15 polydeoxynucleotides + H2O
-
 Escherichia coli hypoxanthine + ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.2.15 56
-
 half-life of 3 min when heated at 56°C Escherichia coli