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Literature summary extracted from

  • Sakamoto, T.; Yamada, M.; Kawasaki, H.; Sakai, T.
    Molecular cloning and nucleotide sequence of an endo-1,5-alpha-L-arabinase gene from Bacillus subtilis (1997), Eur. J. Biochem., 245, 708-714.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.99 analysis useful in structural analysis of primary cell wall polysaccharides Bacillus subtilis
3.2.1.99 nutrition useful for pectin extraction from citrus peel from fruit-juice industries and sugar-beet pulp from sugar factories Bacillus subtilis
3.2.1.99 synthesis applied to the refinement of cotton fiber, enzyme is able to release the cotton fiber coating, yielding product of high quality but with lower amounts of wastes Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.99 IFO 3134 Bacillus subtilis

General Stability

EC Number General Stability Organism
3.2.1.99 IFO 3134, enzyme stable against protease secreted by the microorganism even during 2 days cultivation Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.99 CH3COO- NH4+ IFO 3134, complete inhibition of enzyme production Bacillus subtilis
3.2.1.99 CH3COONa IFO 3134, inhibition of enzyme production Bacillus subtilis
3.2.1.99 additional information IFO 3134, inhibition of enzyme production Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.99 (NH4)2HPO4 IFO 3134, stimulation of enzyme production and activity Bacillus subtilis
3.2.1.99 (NH4)H2PO4 IFO 3134, stimulation of enzyme production and activity Bacillus subtilis
3.2.1.99 K2HPO4 IFO 3134, stimulation of enzyme production and activity Bacillus subtilis
3.2.1.99 KH2PO4 IFO 3134, stimulation of enzyme production and activity Bacillus subtilis
3.2.1.99 additional information IFO 3134, stimulation of enzyme production by several phosphates, but not by chloride, acetate or sulfate Bacillus subtilis
3.2.1.99 Na2HPO4 IFO 3134, stimulation of enzyme production and activity Bacillus subtilis
3.2.1.99 NaH2PO4 IFO 3134, stimulation of enzyme production and activity Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.99 30000
-
 IFO 3134, SDS-PAGE Bacillus subtilis
3.2.1.99 35710
-
 IFO 3134, calculated from nucleotide sequence Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.99 Bacillus subtilis
-
 IFO 3134
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O = alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose regulation, induction and carbon catabolite repression of endo-1,5-alpha-arabinanase Bacillus subtilis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.99 1,5-alpha-L-arabinan + H2O IFO 3134, linear 1,5-alpha-L-arabinan Bacillus subtilis alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
-
 ?
3.2.1.99 L-arabinan + H2O sugar beet pulp Bacillus subtilis L-arabino-oligosaccharides
-
 ?
3.2.1.99 L-arabinan + H2O random hydrolysis of L-arabinan Bacillus subtilis L-arabino-oligosaccharides
-
 ?
3.2.1.99 protopectin + H2O IFO 3134, protopectin of sugar-beet and apple pulp Bacillus subtilis pectin IFO 3134, soluble pectin ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.99 protopectinase C IFO 3134 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.99 60
-
 IFO 3134 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.99 6
-
 IFO 3134 Bacillus subtilis