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Literature summary extracted from

  • Fujisaki, M.; Tsuchida, T.; Kadowaki, S.; Yamamoto, K.; Tochikura, T.
    Purification and properties of Acinetobacter sp. Endo-beta-N-acetylglucosaminidase acting on complex oligosaccharides of glycoproteins (1991), J. Ferment. Bioeng., 71, 242-247.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.96 Hg2+ 1 mM HgCl2, about 50% inhibition Acinetobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.96 0.68
-
 dansyl asialotransferrin glycopeptide
-
 Acinetobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.96 35000
-
 gel filtration Acinetobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.96 Acinetobacter sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.96
-
 Acinetobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.96 additional information
-
-
 Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.96 dansyl asialotransferrin glycopeptide + H2O
-
 Acinetobacter sp. ?
-
 ?
3.2.1.96 glycopeptide + H2O high activity on dansyl transferrin glycopeptide, can also hydrolyze dansyl asialotransferrin glycopeptide and dansyl transferrin glycopeptide containing complex type sugar chains Acinetobacter sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.96 monomer 1 * 35000, SDS-PAGE Acinetobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.96 50
-
-
 Acinetobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.96 45
-
 10 min, 10 mM phosphate buffer, pH 7.0, stable below Acinetobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.96 3 3.5
-
 Acinetobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.96 2.5 5 about 30% of maxiaml activity at pH 2.5 and at pH 5.0 Acinetobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.96 6 8 4°C, 5 d, stable Acinetobacter sp.