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Literature summary extracted from

  • Koide, N.; Muramatsu, T.
    endo-beta-N-Acetylglucosaminidase acting on carbohydrate moieties of glycoproteins. Purification and properties of the enzyme from Diplococcus pneumoniae (1974), J. Biol. Chem., 249, 4897-4904.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.96 N-acetylglucosamino(1->5)lactone
-
 Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.96 0.25
-
 Asn(GlcNAc)2-(Man)5
-
 Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.96 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.96
-
 Streptococcus pneumoniae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.96 culture medium
-
 Streptococcus pneumoniae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.96 4.67
-
-
 Streptococcus pneumoniae

Storage Stability

EC Number Storage Stability Organism
3.2.1.96 -20°C, protein concentration more than 0.5 mg/ml, less than 30% loss of activity after 2 months Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.96 Asn(GlcNAc)2-(Man)5 + H2O
-
 Streptococcus pneumoniae AsnGlcNAc + Man5(GlcNAc)
-
 ?
3.2.1.96 glycopeptide + H2O specificity Streptococcus pneumoniae ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.96 6.5
-
 hydrolysis of Asn(GlcNAc)2-(Man)5 Streptococcus pneumoniae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.96 5.3 8.2 about 50% of maximal activity at pH 5.3 and at pH 8.2 Streptococcus pneumoniae