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Literature summary extracted from

  • Chalifour, R.J.; Spiro, R.G.
    Cleavage of dolichyl pyrophosphoryl oligosaccharides by endo-beta-N-acetylglucosaminidase H: comparison of enzymatic and acid hydrolysis techniques for saccharide release (1984), Arch. Biochem. Biophys., 229, 386-394.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.96 Triton X-100 maximal stimulation at 0.03%, or small amounts of other detergents Streptomyces griseus

Application

EC Number Application Comment Organism
3.2.1.96 analysis useful in metabolic studies dealing with oligosaccharide-lipid assembly and their involvement in the N-glycosylation of proteins Streptomyces griseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.96 SDS 0.1%, strong inhibition Streptomyces griseus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.96 Streptomyces griseus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.96 dolichyl pyrophosphoryl oligosaccharides + H2O both glucosylated and unglucosylated polymannose oligosaccharides are released by the enzyme through cleavage of the di-N-acetylchitobiose squence Streptomyces griseus ?
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 ?
3.2.1.96 additional information specificity Streptomyces griseus ?
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 ?
3.2.1.96 additional information the rate of hydrolysis of lipid-linked (Glc)3(Man)9(GlcNAc)2 is substantially slower than that of the same oligosaccharide attached to asparagine in a peptide sequence Streptomyces griseus ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.96 endo-beta-N-acetylglucosaminidase H
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 Streptomyces griseus