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Literature summary extracted from

  • Fukumura, M.K.; Sakka, K.; Shimida, K.; Ohmiya, K.
    Nucleotide sequence of the Clostridium stercorarium xynB gene encoding an extremely thermostable xylanase and characterization of the translated product (1995), Biosci. Biotechnol. Biochem., 59, 40-46.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 expression in Escherichia coli Thermoclostridium stercorarium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 41000
-
 x * 41000, SDS-PAGE Thermoclostridium stercorarium
3.2.1.8 44377
-
 x * 44377, calculation from nucleotide sequence Thermoclostridium stercorarium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Thermoclostridium stercorarium
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.8 proteolytic modification 32 amino acids are removed from the N-terminus of Xyn B by proteolysis, resulting in the formation of a protein species with a predicted molecular weight of 40805 Da Thermoclostridium stercorarium

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
 Thermoclostridium stercorarium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 additional information
-
-
 Thermoclostridium stercorarium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 1,4-beta-D-xylan + H2O
-
 Thermoclostridium stercorarium additional information
-
 ?
3.2.1.8 carboxymethylcellulose + H2O
-
 Thermoclostridium stercorarium ?
-
 ?
3.2.1.8 p-nitrophenyl beta-D-cellobioside + H2O
-
 Thermoclostridium stercorarium ?
-
 ?
3.2.1.8 p-nitrophenyl beta-D-xylopyranoside
-
 Thermoclostridium stercorarium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 41000, SDS-PAGE Thermoclostridium stercorarium
3.2.1.8 ? x * 44377, calculation from nucleotide sequence Thermoclostridium stercorarium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 80
-
 hydrolysis of xylan Thermoclostridium stercorarium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 60 90 60°C: about 50% of maximal activity, 90°C: about 20% of maximal activity Thermoclostridium stercorarium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 70
-
 stable up to Thermoclostridium stercorarium
3.2.1.8 100
-
 10 min, retains 50% of its activity at pH 6.1, retains 58% of its activity at pH 6.4, completly inactivated at pH 5.8 Thermoclostridium stercorarium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6
-
-
 Thermoclostridium stercorarium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 5 8 pH 5.0: about 80% of maximal activity, pH 8.0: about 65% of maximal activity Thermoclostridium stercorarium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 5 12 4°C, 24 h, stable Thermoclostridium stercorarium