Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hahn, M.; Olsen, O.; Politz, O.; Borriss, R.; Heinemann, U.
    Crystal structure and site-directed mutagenesis of Bacillus macerans endo-1,3-1,4-beta-glucanase (1995), J. Biol. Chem., 270, 3081-3088.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.73
-
 Paenibacillus macerans

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.73 D105K activity is reduced to less than 1% Paenibacillus macerans
3.2.1.73 D105N activity is reduced to less than 1% Paenibacillus macerans
3.2.1.73 E103D activity is reduced to less than 1% Paenibacillus macerans
3.2.1.73 E103Q activity is reduced to less than 1% Paenibacillus macerans
3.2.1.73 E107D activity is reduced to less than 1% Paenibacillus macerans
3.2.1.73 E107H activity is reduced to less than 1% Paenibacillus macerans
3.2.1.73 E107Q activity is reduced to less than 1% Paenibacillus macerans
3.2.1.73 W101F activity is reduced to less than 1% Paenibacillus macerans
3.2.1.73 W101Y activity is reduced to less than 1% Paenibacillus macerans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.73 additional information
-
 additional information wild-type and mutant enzymes Paenibacillus macerans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Paenibacillus macerans
-
 wild-type and mutant enzymes W101F, W101Y, E103D, E103Q, D105N, D105K, E107D, E107Q, and E107H
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 wild type and mutant enzymes W101F, W101Y, E103D, E103Q, D105N, D105K, E107D, E107Q, and E107H Paenibacillus macerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 lichenan + H2O
-
 Paenibacillus macerans ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.73 0.2
-
 lichenan mutant enzyme E107D Paenibacillus macerans
3.2.1.73 0.55
-
 lichenan mutant enzyme E103D Paenibacillus macerans
3.2.1.73 0.7
-
 lichenan mutant enzyme E107H Paenibacillus macerans
3.2.1.73 1.7
-
 lichenan mutant enzyme W101Y Paenibacillus macerans
3.2.1.73 2.9
-
 lichenan mutant enzyme W101F Paenibacillus macerans
3.2.1.73 3.1
-
 lichenan mutant enzyme D105N Paenibacillus macerans
3.2.1.73 1020
-
 lichenan wild-type enzyme Paenibacillus macerans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.73 5.5 6 mutant enzyme E107H Paenibacillus macerans
3.2.1.73 6 7 wild-type enzyme and mutant enzymes W101F, W101Y, E103D, D105N and E107D Paenibacillus macerans