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Literature summary extracted from
- Purification and partial characterization of hyaluronidase from stonefish (Synanceja horrida) venom (1992), Comp. Biochem. Physiol. B, 101, 159-163.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.35 | extremely labile, addition of 1% bovine serum albumin prevents inactivation for more than 1 month at -70°C | Synanceia horrida |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.35 | Cu2+ | 67% inhibition at 5 mM | Synanceia horrida |  |
| 3.2.1.35 | heparin | 65% inhibition at 5 mM | Synanceia horrida | |
| 3.2.1.35 | Hg2+ | 43% inhibition at 5 mM | Synanceia horrida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.35 | additional information | - |
hyaluronoic acid | value in mg/ml | Synanceia horrida | |
| 3.2.1.35 | additional information | - |
hyaluronoic acid | 0.71 mg/ml | Synanceia horrida |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.35 | extracellular | from venom sacs | Synanceia horrida | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.35 | 62000 | - |
SDS-PAGE, gel filration | Synanceia horrida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.35 | hyaluronic acid + H2O | Synanceia horrida | spreading factor for dermonecrosis | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.35 | Synanceia horrida | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.35 | glycoprotein | - |
Synanceia horrida |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.35 | to homogeneity, 2step chromatography | Synanceia horrida |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.35 | spinal column | - |
Synanceia horrida | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.35 | additional information | - |
activity measured in national formulary units | Synanceia horrida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.35 | hyaluronic acid + H2O | spreading factor for dermonecrosis | Synanceia horrida | ? | - |
? | |
| 3.2.1.35 | hyaluronic acid + H2O | - |
Synanceia horrida | hyaluronic acid oligomers | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.35 | monomer | 1 * 62000, SDS-PAGE | Synanceia horrida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.35 | 37 | 40 | - |
Synanceia horrida |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.35 | 26 | 50 | - |
Synanceia horrida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.35 | 6 | - |
- |
Synanceia horrida |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.35 | 5 | 9 | - |
Synanceia horrida |
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