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Literature summary extracted from

  • Toida, T.; Ogita, Y.; Suzuki, A.; Toyoda, H.; Imanari, T.
    Inhibition of hyaluronidase by fully O-sulfonated glycosaminoglycans (1999), Arch. Biochem. Biophys., 370, 176-182.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.35 chondroitin O-sulfate IC50 = 0.00133 mg/ml Homo sapiens
3.2.1.35 dermatan O-sulfate IC50 = 0.00133 mg/ml Homo sapiens
3.2.1.35 fully O-sulfonated chondroitin sulfate competitive and noncompetitive mode, 50% inhibition at 0.00135 mg/ml Homo sapiens
3.2.1.35 fully O-sulfonated dermatan sulfate competitive and noncompetitive mode, 50% inhibition at 0.00133 mg/ml Homo sapiens
3.2.1.35 fully O-sulfonated heparan sulfate competitive and noncompetitive mode, 50% inhibition at 0.00128 mg/ml Homo sapiens
3.2.1.35 fully O-sulfonated heparin 50% inhibition at 0.00114 mg/ml Homo sapiens
3.2.1.35 fully O-sulfonated hyaluronan 50% inhibition at 0.00078 mg/ml Homo sapiens
3.2.1.35 heparan O-sulfate IC50 = 0.00128 mg/ml Homo sapiens
3.2.1.35 heparin IC50 = 0.00114 mg/ml, non-competitive inhibition; noncompetitive Homo sapiens
3.2.1.35 heparin O-sulfate IC50 = 0.00114 mg/ml, competitive and non-competitive inhibitory effects Homo sapiens
3.2.1.35 hyaluronan O-sulfate IC50 = 0.00078 mg/ml Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.35 extracellular
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.35 hyaluronic acid + H2O Homo sapiens involved in inflammatory reactions, exists as inactive form activated by metal ions ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.35 hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates, and dermatan Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.35 urine
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.35 additional information
-
 activity measured as turbidicity reducing units Homo sapiens
3.2.1.35 additional information
-
 development of a flow injection assay method using commercial bovine enzyme and fluorometric detection, comparison of fluorogenic reagents, enzyme activity in human urine samples Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 hyaluronan + 2 H2O from Streptococcus zooepidemicus Homo sapiens N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
 ?
3.2.1.35 hyaluronan + H2O
-
 Homo sapiens tetraoligosaccharides + hexaoligosaccharides after complete digestion ?
3.2.1.35 hyaluronic acid + H2O involved in inflammatory reactions, exists as inactive form activated by metal ions Homo sapiens ?
-
 ?
3.2.1.35 hyaluronic acid + H2O
-
 Homo sapiens hyaluronic acid oligomers
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.35 HAase
-
 Homo sapiens
3.2.1.35 hyaluronidase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.35 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.35 5
-
 assay at Homo sapiens