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Literature summary extracted from

  • Lee, E.Y.C.; Carter, J.H.; Nielsen, L.D.; Fischer, E.H.
    Purification and properties of yeast amylo-1,6-glucosidase-oligo-1,4 -> 1,4-glucantransferase (1970), Biochemistry, 9, 2347-2355.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.33 210000
-
 glycogen debranching enzyme, gel filtration and PAGE Saccharomyces cerevisiae
3.2.1.33 280000
-
 glycogen debranching enzyme, sedimentation equilibrium Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O Saccharomyces cerevisiae
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.33 Saccharomyces cerevisiae
-
 baker's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.33 glycogen debranching enzyme Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.33 6
-
-
 Saccharomyces cerevisiae
3.2.1.33 8.4
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.33 alpha-(1-6)-glucosyl cycloheptaamylose + H2O 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin Saccharomyces cerevisiae cycloheptaamylose + D-glucose alpha-Schardinger dextrin r
3.2.1.33 alpha-(1-6)-glucosyl cyclohexaamylose + H2O 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin Saccharomyces cerevisiae cyclohexaamylose + D-glucose alpha-Schardinger dextrin r
3.2.1.33 amylopectin + H2O slowly hydrolyzed Saccharomyces cerevisiae amylopectin + D-glucose
-
 r
3.2.1.33 D-glucose + maltooligosaccharide maltotetraose and maltopentaose Saccharomyces cerevisiae maltooligosaccharide branched r
3.2.1.33 glycogen + H2O native glycogen from shellfish and rabbit liver Saccharomyces cerevisiae glycogen + D-glucose
-
 r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O
-
 Saccharomyces cerevisiae limit dextrin + D-glucose
-
 r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O
-
 Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.33 trimer
-
 Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.33 glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.33 6 6.6
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.33 5 8 pH 5.0: about 25% of maximum activity, pH 8.0: about 40% of maximum activity Saccharomyces cerevisiae