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Literature summary extracted from

  • Shaikh, S.A.; Khire, J.M.; Khan, K.M.I.
    Characterization of a thermostable extracellular beta-galactosidase from a thermophilic fungus Rhizomucor sp. (1999), Biochim. Biophys. Acta, 1472, 314-322.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.23 nutrition potential use of the enzyme in the hydrolysis of whey and of milk lactose Rhizomucor sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Cu2+
-
 Rhizomucor sp.
3.2.1.23 D-galactose
-
 Rhizomucor sp.
3.2.1.23 Hg2+
-
 Rhizomucor sp.
3.2.1.23 isopropyl thiogalactoside
-
 Rhizomucor sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.66
-
 p-nitrophenyl beta-D-galactopyranoside
-
 Rhizomucor sp.
3.2.1.23 1.32
-
 o-nitrophenyl-beta-D-galactoside
-
 Rhizomucor sp.
3.2.1.23 50
-
 lactose
-
 Rhizomucor sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.23 extracellular
-
 Rhizomucor sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 120000
-
 2 * 120000, SDS-PAGE Rhizomucor sp.
3.2.1.23 250000
-
 gel filtration Rhizomucor sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Rhizomucor sp.
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.23 glycoprotein contains 8% neutral sugar Rhizomucor sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Rhizomucor sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 200
-
-
 Rhizomucor sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O
-
 Rhizomucor sp. D-glucose + D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Rhizomucor sp. o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Rhizomucor sp. p-nitrophenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 dimer 2 * 120000, SDS-PAGE Rhizomucor sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 60
-
-
 Rhizomucor sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 60
-
 4 h, stable Rhizomucor sp.
3.2.1.23 70
-
 half-life: 150 min Rhizomucor sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 4.5
-
-
 Rhizomucor sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 3.5 7.5 stable Rhizomucor sp.