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Literature summary extracted from

  • Ohtsu, N.; Motoshima, H.; Goto, K.; Tsukasaki, F.; Matsuzawa, H.
    Thermostable beta-galactosidase from an extreme thermophile, Thermus sp. A4: enzyme purification and characterization, and gene cloning and sequencing (1998), Biosci. Biotechnol. Biochem., 62, 1539-1545.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.23 2-mercaptoethanol
-
 Thermus sp.
3.2.1.23 Cys activates Thermus sp.
3.2.1.23 dithiothreitol activates Thermus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.23
-
 Thermus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Cu2+ 10 mM, 32% inhibition Thermus sp.
3.2.1.23 Fe2+ 10 mM, 16% inhibition Thermus sp.
3.2.1.23 PCMB
-
 Thermus sp.
3.2.1.23 phenylmethylsulfonyl fluoride
-
 Thermus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 5.9
-
 o-nitrophenyl-beta-D-galactoside
-
 Thermus sp.
3.2.1.23 19
-
 lactose
-
 Thermus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 Co2+ 10 mM, activates Thermus sp.
3.2.1.23 Mn2+ 10 mM, activates Thermus sp.
3.2.1.23 Zn2+ 10 mM, activates Thermus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 72741
-
 x * 72741, calculation from nucleotide sequence Thermus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Thermus sp.
-
-
-
3.2.1.23 Thermus sp. A4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Thermus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 48.6
-
-
 Thermus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O
-
 Thermus sp. D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Thermus sp. A4 D-glucose + D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Thermus sp. o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Thermus sp. A4 o-nitrophenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 ? x * 72741, calculation from nucleotide sequence Thermus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 70
-
 20 h, stable Thermus sp.
3.2.1.23 85
-
 2 h, retains more than 75% of its activity Thermus sp.
3.2.1.23 90
-
 half-life: 1 h Thermus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.23 12.5
-
 lactose
-
 Thermus sp.
3.2.1.23 66.7
-
 o-nitrophenyl-beta-D-galactoside
-
 Thermus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 6.5
-
-
 Thermus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 5 8 pH 5.0: about 30% of maximal activity, pH 8.5: about 50% of maximal activity Thermus sp.