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Literature summary extracted from

  • Goodman, R.E.; Pederson, D.M.
    beta-Galactosidase from Bacillus stearothermophilus (1976), Can. J. Microbiol., 22, 817-825.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 cellobiose 10 mM, 33% inhibition Geobacillus stearothermophilus
3.2.1.23 Cu2+ 0.1 mM, 92% inhibition Geobacillus stearothermophilus
3.2.1.23 D-galactose 10 mM, 71% inhibition, product inhibition Geobacillus stearothermophilus
3.2.1.23 D-glucose 10 mM, 20% inhibition Geobacillus stearothermophilus
3.2.1.23 Zn2+ 0.1 mM ZnCl2, 30% inhibition Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.11
-
 lactose
-
 Geobacillus stearothermophilus
3.2.1.23 9.8
-
 o-nitrophenyl-beta-D-galactoside
-
 Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 215000
-
-
 Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.23 additional information Geobacillus stearothermophilus constitutive enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23 partial Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O
-
 Geobacillus stearothermophilus D-glucose + D-galactose
-
 ?
3.2.1.23 additional information constitutive enzyme Geobacillus stearothermophilus ?
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Geobacillus stearothermophilus o-nitrophenol + beta-D-galactose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 65
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 50
-
 half-life: 20 days Geobacillus stearothermophilus
3.2.1.23 60
-
 10 min, more than 60% loss of activity, 18% loss of activity in presence of 4 mg/ml bovine serum albumin Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 6 6.4 hydrolysis of lactose and o-nitrophenyl-beta-D-galactoside Geobacillus stearothermophilus