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Literature summary extracted from

  • Watanabe, Y.; Kibesaki, Y.; Takenishi, S.; Sakai, K.; Tsujisaka, Y.
    Purification and properties of beta-galactosidase from Penicillium citrinum (1979), Agric. Biol. Chem., 43, 943-950.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Cu2+
-
 Aspergillus oryzae
3.2.1.23 Cu2+ 1 mM, 59% inhibition Penicillium citrinum
3.2.1.23 Hg2+
-
 Aspergillus oryzae
3.2.1.23 Hg2+ 1 mM, complete inhibition Penicillium citrinum
3.2.1.23 additional information no substrate inhibition and no product inhibition Penicillium citrinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 1.7
-
 o-nitrophenyl-beta-D-galactoside
-
 Penicillium citrinum
3.2.1.23 1.7
-
 p-nitrophenyl-beta-D-galactoside
-
 Penicillium citrinum
3.2.1.23 1.7
-
 phenyl-beta-D-galactoside
-
 Penicillium citrinum
3.2.1.23 25
-
 lactose
-
 Penicillium citrinum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 60000
-
 2 * 60000, SDS-PAGE Penicillium citrinum
3.2.1.23 100000
-
 gel filtration Penicillium citrinum
3.2.1.23 110000
-
 equilibrium sedimentation Penicillium citrinum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Ascospirella lutea
-
-
-
3.2.1.23 Aspergillus awamori
-
-
-
3.2.1.23 Aspergillus cellulosae
-
-
-
3.2.1.23 Aspergillus niger
-
-
-
3.2.1.23 Aspergillus oryzae
-
-
-
3.2.1.23 Fusarium oxysporum
-
-
-
3.2.1.23 Geotrichum candidum
-
-
-
3.2.1.23 Mucor javanicus
-
-
-
3.2.1.23 Mucor mucedo
-
-
-
3.2.1.23 Neurospora crassa
-
-
-
3.2.1.23 Penicillium chrysogenum
-
-
-
3.2.1.23 Penicillium citrinum
-
-
-
3.2.1.23 Penicillium cyclopium
-
-
-
3.2.1.23 Penicillium glabrum
-
-
-
3.2.1.23 Penicillium glaucum
-
-
-
3.2.1.23 Penicillium roqueforti
-
-
-
3.2.1.23 Penicillium toxidarium
-
-
-
3.2.1.23 Rhizopus acidus
-
-
-
3.2.1.23 Rhizopus arrhizus
-
-
-
3.2.1.23 Rhizopus formosciensis
-
-
-
3.2.1.23 Rhizopus microsporus var. chinensis
-
-
-
3.2.1.23 Rhizopus niveus
-
-
-
3.2.1.23 Rhizopus stolonifer
-
-
-
3.2.1.23 Trichoderma viride
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.23 glycoprotein
-
 Neurospora crassa
3.2.1.23 glycoprotein
-
 Aspergillus niger
3.2.1.23 glycoprotein contains 5.8% carbohydrate Penicillium citrinum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Penicillium citrinum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 98.1
-
-
 Penicillium citrinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O
-
 Aspergillus niger D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Aspergillus oryzae D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Penicillium citrinum D-glucose + D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Neurospora crassa o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Aspergillus niger o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Aspergillus oryzae o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Penicillium citrinum o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Aspergillus niger p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Penicillium citrinum p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 phenyl-beta-D-galactoside + H2O
-
 Aspergillus oryzae phenol + beta-D-galactose
-
 ?
3.2.1.23 phenyl-beta-D-galactoside + H2O
-
 Penicillium citrinum phenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 dimer 2 * 60000, SDS-PAGE Penicillium citrinum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 50
-
 pH 4.5, 15 min Penicillium citrinum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 40
-
-
 Aspergillus oryzae
3.2.1.23 40
-
 pH 5.6, 15 min, stable below Penicillium citrinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 4.5
-
-
 Neurospora crassa
3.2.1.23 4.5
-
-
 Aspergillus oryzae
3.2.1.23 4.5
-
 at 40°C, 15 min Penicillium citrinum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 4.5 7 40°C, 3 h, stable Penicillium citrinum