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Literature summary extracted from
- beta-Galactosidase from rat brain (1969), Methods Enzymol., 14, 156-161.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.23 | sodium cholate | required for hydrolysis of lipid substrates | Rattus norvegicus |  |
| 3.2.1.23 | taurocholate | required for hydrolysis of lipid substrates | Rattus norvegicus | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.23 | ceramide | hydrolysis of lactosyl ceramide | Rattus norvegicus | |
| 3.2.1.23 | D-galactono-1,4-lactone | hydrolysis of lactosyl ceramide and p-nitrophenyl galactoside | Rattus norvegicus | |
| 3.2.1.23 | sphingosine | hydrolysis of lactosyl ceramide | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.23 | 0.02 | - |
lactosyldiceramide | - |
Rattus norvegicus | |
| 3.2.1.23 | 0.1 | - |
tetrahexosyldiceramide | - |
Rattus norvegicus | |
| 3.2.1.23 | 0.3 | - |
monosialoganglioside | - |
Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.23 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.23 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.23 | brain | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 11.83 | - |
- |
Rattus norvegicus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.23 | -20°C, stable for at least 6 months | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.23 | digalactosyl ceramide + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.2.1.23 | lactose + H2O | - |
Rattus norvegicus | D-glucose + D-galactose | - |
? | |
| 3.2.1.23 | lactosyl ceramide + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.2.1.23 | lactosyldiceramide | - |
Rattus norvegicus | ? | - |
? | |
| 3.2.1.23 | monosialoganglioside + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.2.1.23 | o-nitrophenyl-beta-D-galactoside + H2O | - |
Rattus norvegicus | o-nitrophenol + beta-D-galactose | - |
? | |
| 3.2.1.23 | p-nitrophenyl-beta-D-galactoside + H2O | - |
Rattus norvegicus | p-nitrophenol + beta-D-galactose | - |
? | |
| 3.2.1.23 | tetrahexosyl ceramide + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.2.1.23 | tetrahexosyldiceramide | - |
Rattus norvegicus | ? | - |
? | |
| 3.2.1.23 | trihexosyl ceramide + H2O | - |
Rattus norvegicus | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 3.1 | - |
hydrolysis of p-nitrophenyl galactoside | Rattus norvegicus |
| 3.2.1.23 | 5 | - |
hydrolysis of lactosyl ceramide | Rattus norvegicus |
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Release 2023.1 (January 2023)
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