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Literature summary extracted from

  • Itoh, T.; Suzuki, M.; Adachi, S.
    Production and characterization of beta-galactosidase from lactose-fermenting yeasts (1982), Agric. Biol. Chem., 46, 899-904.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Ca2+ 0.2 mM, 55-65% inhibition Kluyveromyces marxianus
3.2.1.23 Ca2+
-
 Torulopsis sphaerica
3.2.1.23 Ca2+ 0.2 mM, 55-65% inhibition Wickerhamiella versatilis
3.2.1.23 cellobiose slight inhibition Kluyveromyces marxianus
3.2.1.23 cellobiose slight inhibition Torulopsis sphaerica
3.2.1.23 cellobiose slight inhibition Wickerhamiella versatilis
3.2.1.23 D-galactose slight inhibition Kluyveromyces marxianus
3.2.1.23 D-galactose slight inhibition Torulopsis sphaerica
3.2.1.23 D-galactose slight inhibition Wickerhamiella versatilis
3.2.1.23 D-glucose slight inhibition Kluyveromyces marxianus
3.2.1.23 D-glucose slight inhibition Torulopsis sphaerica
3.2.1.23 D-glucose slight inhibition Wickerhamiella versatilis
3.2.1.23 fructose slight inhibition Kluyveromyces marxianus
3.2.1.23 fructose slight inhibition Torulopsis sphaerica
3.2.1.23 fructose slight inhibition Wickerhamiella versatilis
3.2.1.23 Hg2+ 0.2 mM, complete inhibition Kluyveromyces marxianus
3.2.1.23 Hg2+ 0.2 mM, complete inhibition Torulopsis sphaerica
3.2.1.23 Hg2+
-
 Wickerhamiella versatilis
3.2.1.23 iodoacetamide
-
 Kluyveromyces marxianus
3.2.1.23 iodoacetamide
-
 Torulopsis sphaerica
3.2.1.23 iodoacetamide
-
 Wickerhamiella versatilis
3.2.1.23 maltose slight inhibition Kluyveromyces marxianus
3.2.1.23 maltose slight inhibition Torulopsis sphaerica
3.2.1.23 maltose slight inhibition Wickerhamiella versatilis
3.2.1.23 Mg2+ weak Kluyveromyces marxianus
3.2.1.23 Mg2+
-
 Torulopsis sphaerica
3.2.1.23 Mg2+ weak Wickerhamiella versatilis
3.2.1.23 NEM
-
 Kluyveromyces marxianus
3.2.1.23 NEM
-
 Torulopsis sphaerica
3.2.1.23 NEM
-
 Wickerhamiella versatilis
3.2.1.23 sucrose slight inhibition Kluyveromyces marxianus
3.2.1.23 sucrose slight inhibition Torulopsis sphaerica
3.2.1.23 sucrose slight inhibition Wickerhamiella versatilis
3.2.1.23 xylose slight inhibition Torulopsis sphaerica
3.2.1.23 xylose slight inhibition Wickerhamiella versatilis
3.2.1.23 Zn2+ 0.2 mM Kluyveromyces marxianus
3.2.1.23 Zn2+
-
 Torulopsis sphaerica
3.2.1.23 Zn2+ 0.2 mM Wickerhamiella versatilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 2.09
-
 o-nitrophenyl-beta-D-galactoside
-
 Torulopsis sphaerica
3.2.1.23 2.82
-
 o-nitrophenyl-beta-D-galactoside strain A60 Kluyveromyces marxianus
3.2.1.23 3.45
-
 o-nitrophenyl-beta-D-galactoside strain B57 Kluyveromyces marxianus
3.2.1.23 3.45
-
 o-nitrophenyl-beta-D-galactoside strain M6 Wickerhamiella versatilis
3.2.1.23 16.7
-
 lactose strain A60 Kluyveromyces marxianus
3.2.1.23 25
-
 lactose
-
 Torulopsis sphaerica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 Mn2+ 0.2 mM, slight activation Kluyveromyces marxianus
3.2.1.23 Mn2+ 0.2 mM, slight activation Wickerhamiella versatilis
3.2.1.23 Mn2+ 0.2 mM, slight activation Torulopsis sphaerica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 200000
-
 strain B57, gel filtration Kluyveromyces marxianus
3.2.1.23 215000
-
 strain A60, gel filtration Kluyveromyces marxianus
3.2.1.23 226000
-
 gel filtration Wickerhamiella versatilis
3.2.1.23 233000
-
 gel filtration Torulopsis sphaerica

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Kluyveromyces marxianus
-
 and strain A60
-
3.2.1.23 Kluyveromyces marxianus B57
-
 and strain A60
-
3.2.1.23 Torulopsis sphaerica
-
-
-
3.2.1.23 Torulopsis sphaerica J28
-
-
-
3.2.1.23 Wickerhamiella versatilis
-
-
-
3.2.1.23 Wickerhamiella versatilis M6
-
-
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.23 4°C, lyophilized preparation, stable for 6 months Kluyveromyces marxianus
3.2.1.23 4°C, lyophilized preparation, stable for 6 months Wickerhamiella versatilis
3.2.1.23 4°C, lyophilized preparation, stable for 6 months Torulopsis sphaerica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O
-
 Torulopsis sphaerica D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Torulopsis sphaerica J28 D-galactose + D-glucose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Kluyveromyces marxianus o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Wickerhamiella versatilis o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Torulopsis sphaerica o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Torulopsis sphaerica J28 o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Wickerhamiella versatilis M6 o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Kluyveromyces marxianus B57 o-nitrophenol + beta-D-galactose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 30
-
-
 Kluyveromyces marxianus
3.2.1.23 30
-
-
 Wickerhamiella versatilis
3.2.1.23 30
-
-
 Torulopsis sphaerica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 35
-
 10 min, 50% loss of activity Kluyveromyces marxianus
3.2.1.23 35
-
 10 min, 50% loss of activity Wickerhamiella versatilis
3.2.1.23 35
-
 10 min, 50% loss of activity Torulopsis sphaerica
3.2.1.23 45
-
 10 min, complete inactivation Kluyveromyces marxianus
3.2.1.23 45
-
 10 min, complete inactivation Wickerhamiella versatilis
3.2.1.23 45
-
 10 min, complete inactivation Torulopsis sphaerica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 7
-
-
 Wickerhamiella versatilis
3.2.1.23 7
-
-
 Torulopsis sphaerica
3.2.1.23 7.5
-
-
 Kluyveromyces marxianus