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Literature summary extracted from

  • Nakanishi, K.; Matsuno, R.; Torii, K.; Yamamoto, K.; Kamikubo, T.
    Properties of immobilized beta-D-galactosidase from Bacillus circulans (1983), Enzyme Microb. Technol., 5, 115-120.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 oligosaccharides oligosaccharides which are produced as a by-product in the hydrolysis of lactose promote a reversible inactivation of the immobilized enzyme. The inactivation can be reduced by having the reaction in a continuous stirred reactor at a high percent conversion of lactose or by using any immobilized enzyme having a low specific activity Niallia circulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 41.7
-
 lactose
-
 Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Niallia circulans
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 additional information
-
 highest activity for hydrolysis of lactose is obtained with immobilization onto Duolite ES-762 Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O
-
 Niallia circulans D-glucose + D-galactose glucose + galactose + considerable amounts of trisaccharides and tetrasaccharides + trace amounts of oligosaccharides ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 60
-
 hydrolysis of lactose in buffer solution Niallia circulans
3.2.1.23 65
-
 hydrolysis of lactose in skim milk Niallia circulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 50
-
 half-life of immobilized enzyme in a continuous stirred reactor: 50 days Niallia circulans
3.2.1.23 55
-
 half-life of immobilized enzyme in a continuous stirred reactor: 15 days Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 6
-
 hydrolysis of lactose Niallia circulans
3.2.1.23 6.5 8.2 immobilized enzyme Niallia circulans