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Literature summary extracted from
- Purification and characterization of cytosolic sialidase from rat liver (1986), J. Biol. Chem., 260, 6710-6716.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.18 | 0.67 | - |
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | - |
Rattus norvegicus |  |
| 3.2.1.18 | 1.47 | - |
colominic acid | - |
Rattus norvegicus | |
| 3.2.1.18 | 1.67 | - |
sialyl-alpha-2,3-lactose | - |
Rattus norvegicus | |
| 3.2.1.18 | 1.74 | - |
orosomucoid | - |
Rattus norvegicus | |
| 3.2.1.18 | 1.75 | - |
GD1a | - |
Rattus norvegicus | |
| 3.2.1.18 | 1.79 | - |
transferrin | - |
Rattus norvegicus | |
| 3.2.1.18 | 1.91 | - |
sialyl-alpha-2,6-lactose | - |
Rattus norvegicus | |
| 3.2.1.18 | 2.1 | - |
fetuin | - |
Rattus norvegicus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.18 | cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.18 | 43000 | - |
sucrose density gradient centrifugation | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.18 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.18 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.18 | 308 | - |
- |
Rattus norvegicus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.18 | -80°C, one month | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.18 | colominic acid + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.2.1.18 | fetuin + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.2.1.18 | ganglioside GD1a + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.2.1.18 | ganglioside GD1b + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.2.1.18 | orosomucoid + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.2.1.18 | sialyl-alpha-2,3-lactose + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.2.1.18 | sialyl-alpha-2,6-lactose + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.2.1.18 | transferrin + H2O | - |
Rattus norvegicus | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.18 | 6 | - |
orosomucoid as substrate | Rattus norvegicus |
| 3.2.1.18 | 6.5 | - |
sialyllactose as substrate | Rattus norvegicus |
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