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Literature summary extracted from

  • Sugihara, A.; Shimada, Y.; Nagao, T.; Iizumi, T.; Nakamura, K.; Fukase, T.; Tominaga, Y.
    Purification and characterization of a carboxylesterase from Pseudomonas sp. KWI-56 (1994), Biosci. Biotechnol. Biochem., 58, 752-755.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 overproduction in Escherichia coli Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 DFP
-
 Pseudomonas sp.
3.1.1.1 eserine
-
 Pseudomonas sp.
3.1.1.1 Hg2+ HgCl2 Pseudomonas sp.
3.1.1.1 PCMB
-
 Pseudomonas sp.
3.1.1.1 PMSF
-
 Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 28000
-
 2 * 28000, SDS-PAGE Pseudomonas sp.
3.1.1.1 50000
-
 gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Pseudomonas sp.
-
-
-
3.1.1.1 Pseudomonas sp. KWI-56
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 1110
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 methyl propionate + H2O hydrolyzed at highest rate among the fatty acid methyl esters Pseudomonas sp. methanol + propionate
-
 ?
3.1.1.1 methyl propionate + H2O hydrolyzed at highest rate among the fatty acid methyl esters Pseudomonas sp. KWI-56 methanol + propionate
-
 ?
3.1.1.1 tributyrylglycerol + H2O
-
 Pseudomonas sp. butyrate + glycerol
-
 ?
3.1.1.1 tributyrylglycerol + H2O
-
 Pseudomonas sp. KWI-56 butyrate + glycerol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 dimer 2 * 28000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 22
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 36
-
 30 min, stable up to Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 7.5
-
 20°C Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 6.5 8 pH 6.5: about 60% of maximal activity, pH 8.0: about 70% of maximal activity Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.1 6 7.5 25°C, 20 h, stable Pseudomonas sp.