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Literature summary extracted from

  • Hettkamp, H.; Bause, E.; Legler, G.
    Inhibition by nojirimycin and 1-deoxynojirimycin of microsomal glucosidases from calf liver acting on the glycoprotein oligosaccharides Glc1-3Man9GlcNAc2 (1982), Biosci. Rep., 2, 899-906.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.106 Triton X-100 activation, maximal at 0.5% Triton X-100 Bos taurus
3.2.1.106 Triton X-100 several-fold activation Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.106 1-deoxynojirimycin 0.01 mM: 80% inhibition; strong inhibition Bos taurus
3.2.1.106 additional information no inhibition by conduritol B epoxide, bromoconduritols a and B, acarbose at 1 mM Bos taurus
3.2.1.106 Nojirimycin strong inhibition, 0.1 mM: 40% inhibition, 0.16 mM: 50% inhibition Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.106 endoplasmic reticulum rough endoplasmic reticulum Bos taurus 5783
-
3.2.1.106 membrane membrane-bound Bos taurus 16020
-
3.2.1.106 microsome
-
 Bos taurus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.106 Glc3Man9GlcNAc2 + H2O Bos taurus hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
3.2.1.106 additional information Bos taurus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
3.2.1.106 additional information Bos taurus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
3.2.1.106 additional information Bos taurus glucosidase I is a control enzyme for the regulation of glycoprotein processing ?
-
 ?
3.2.1.106 additional information Bos taurus involved in the formation of high mannose and complex glycoproteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.106 Bos taurus
-
 calf
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.106 Glc3Man9GlcNAc2-[protein] + H2O = Glc2Man9GlcNAc2-[protein] + beta-D-glucopyranose glucosidase I is a specific alpha-(1,2)-glucosidase Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.106 liver calf Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Bos taurus D-glucose + Glc2Man9GlcNAc2
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Bos taurus ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2-lipid + H2O no significant difference in enzyme activity towards free, peptide-bound, or lipid-linked oligosaccharide, lipid- and peptide-linked oligosaccharide are hydrolyzed at a rate only 10% lower than free oligosaccharide Bos taurus D-glucose + Glc2Man9GlcNAc2-lipid
-
 ?
3.2.1.106 Glc3Man9GlcNAc2-peptide + H2O no significant difference in enzyme activity towards free, peptide-bound, or lipid-linked oligosaccharide, lipid- and peptide-linked oligosaccharide are hydrolyzed at a rate only 10% lower than free oligosaccharide Bos taurus D-glucose + Glc2Man9GlcNAc2-peptide
-
 ?
3.2.1.106 additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Bos taurus ?
-
 ?
3.2.1.106 additional information involved in processing of asparagine-linked oligosaccharides Bos taurus ?
-
 ?
3.2.1.106 additional information glucosidase I is a control enzyme for the regulation of glycoprotein processing Bos taurus ?
-
 ?
3.2.1.106 additional information involved in the formation of high mannose and complex glycoproteins Bos taurus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.106 6.2
-
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.106 0.16
-
 Nojirimycin
-
 Bos taurus