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Literature summary extracted from
- Fructose-bisphosphatase from spinach leaf chloroplast and cytoplasm (1982), Methods Enzymol., 90, 371-378.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.11 | fructose 1,6-diphosphate | hysteretic activation of the chloroplastic enzyme | Spinacia oleracea |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.11 | AMP | inhibits cytoplasmic enzyme, no inhibition of chloroplastic enzyme | Spinacia oleracea | |
| 3.1.3.11 | Ca2+ | chloroplastic enzyme | Spinacia oleracea | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.3.11 | chloroplast | - |
Spinacia oleracea | 9507 | - |
| 3.1.3.11 | cytoplasm | - |
Spinacia oleracea | 5737 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.11 | 130000 | - |
cytoplasmic enzyme | Spinacia oleracea |
| 3.1.3.11 | 160000 | - |
chloroplastic enzyme | Spinacia oleracea |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.11 | Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.11 | - |
Spinacia oleracea |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.11 | 62 | - |
cytoplasmic enzyme | Spinacia oleracea |
| 3.1.3.11 | 109 | - |
chloroplastic enzyme | Spinacia oleracea |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.3.11 | -20°C, almost no activity is lost during storage for 6 months, chloroplastic enzyme | Spinacia oleracea |
| 3.1.3.11 | -20°C, stable for 2 weeks, 20% loss of activity after 4 weeks, cytoplasmic enzyme | Spinacia oleracea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.11 | D-fructose 1,6-diphosphate + H2O | - |
Spinacia oleracea | D-fructose 6-phosphate + phosphate | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.11 | 8 | - |
cytoplasmic enzyme | Spinacia oleracea |
| 3.1.3.11 | 8.8 | - |
chloroplastic enzyme | Spinacia oleracea |
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Release 2023.1 (January 2023)
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