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Literature summary extracted from
- Affinity of single- or double-stranded oligodeoxyribonucleotides containing a thymine photodimer for T4 endonuclease V (1989), J. Biol. Chem., 264, 2609-2614.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.25.1 | synthetic gene, expressed in Escherichia coli | Tequatrovirus T4 |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.25.1 | 0.000009 | - |
DNA 18mer containing a TT-dimer | - |
Tequatrovirus T4 | |
| 3.1.25.1 | 0.000019 | - |
DNA 14mer containing a TT-dimer | - |
Tequatrovirus T4 |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.25.1 | 16000 | - |
SDS-PAGE | Tequatrovirus T4 |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.25.1 | DNA + H2O | Tequatrovirus T4 | - |
? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.25.1 | Tequatrovirus T4 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.25.1 | - |
Tequatrovirus T4 |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.1.25.1 | endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | minimum chain length: longer than 10 nucleotides | Tequatrovirus T4 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.25.1 | DNA + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
| 3.1.25.1 | DNA 14mer containing a TT-dimer + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
| 3.1.25.1 | DNA 18mer containing a TT-dimer + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
| 3.1.25.1 | oligodeoxyribonucleotides | UV-irradiated, 14mer and 18mer, single-stranded oligomers are a poorer substrate than the corresponding duplexes | Tequatrovirus T4 | oligonucleotides | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.25.1 | 0.0183 | - |
DNA 18mer containing a TT-dimer | - |
Tequatrovirus T4 | |
| 3.1.25.1 | 0.02 | - |
DNA 14mer containing a TT-dimer | - |
Tequatrovirus T4 |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.25.1 | 6 | 8.5 | pH-optimum of glycosylase activity | Tequatrovirus T4 |
| 3.1.25.1 | 6.5 | - |
pH-optimum of endonuclease activity | Tequatrovirus T4 |
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Release 2023.1 (January 2023)
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