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Literature summary extracted from
- Urea amidolyase of Candida utilis. Characterization of the urea cleavage reactions (1976), Biochim. Biophys. Acta, 438, 574-583.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.3.4.6 | acetamide | - |
Cyberlindnera jadinii |  |
| 6.3.4.6 | ADP | - |
Cyberlindnera jadinii | |
| 6.3.4.6 | Avidin | - |
Cyberlindnera jadinii | |
| 6.3.4.6 | Formamide | - |
Cyberlindnera jadinii | |
| 6.3.4.6 | NaF | - |
Cyberlindnera jadinii | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.3.4.6 | ATP + urea + HCO3- | Cyberlindnera jadinii | enzyme is involved in urea cleavage | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.3.4.6 | Cyberlindnera jadinii | - |
enzymes involved in urea cleavage, biotin carboxylation, urea carboxylation, and allophanate hydrolysis occur as a complex of enzymes | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.3.4.6 | copurification of the enzymes involved in urea cleavage, biotin carboxylation, urea carboxylation, and allophanate hydrolysis occur as a complex of enzymes | Cyberlindnera jadinii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.3.4.6 | ATP + urea + HCO3- | - |
Cyberlindnera jadinii | ADP + phosphate + allophanate | - |
? | |
| 6.3.4.6 | ATP + urea + HCO3- | enzyme is involved in urea cleavage | Cyberlindnera jadinii | ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 6.3.4.6 | 52 | - |
rapid loss of activity | Cyberlindnera jadinii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.3.4.6 | 8 | - |
- |
Cyberlindnera jadinii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.3.4.6 | 7.2 | 9 | pH 7.2: about 50% of maximal activity, pH 9.0: about 30% of maximal activity | Cyberlindnera jadinii |
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Release 2023.1 (January 2023)
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